Recombinant
RabMAb

Recombinant Anti-Prolactin/PRL antibody [EPR19386] (Alexa Fluor® 488) (ab225318)

Overview

  • Product name

    Anti-Prolactin/PRL antibody [EPR19386] (Alexa Fluor® 488)
    See all Prolactin/PRL primary antibodies
  • Description

    Rabbit monoclonal [EPR19386] to Prolactin/PRL (Alexa Fluor® 488)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Mouse Prolactin/PRL aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: P06879

  • Positive control

    • ICC/IF: 293T cells transfected with Prolactin/PRL with Myc-tag
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19386
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab225318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.

This product gave a positive signal in 293T cells transfected with Prolactin with Myc-tag fixed with 4% formaldehyde (10 min)

Target

  • Relevance

    Various hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands.
  • Cellular localization

    Secreted
  • Database links

  • Alternative names

    • Decidual prolactin antibody
    • GHA1 antibody
    • Growth hormone A1 antibody
    • Lactogenic hormone antibody
    • Luteotropic hormone antibody
    • Mammotropin antibody
    • PRL antibody
    • Prolactin antibody
    • Prolactin precursor antibody
    see all

Images

  • ab225318 staining Prolactin/PRL in 293T cells transfected with Prolactin/PRL with Myc-tag. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225318 at 1/200 dilution (shown in green) and ab223894, Mouse monoclonal to Myc-tag (Alexa Fluor® 594), at 1/100 dilution (pseudocolored in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab225318 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab225318.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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