Product nameProlactin/PRL overexpression 293T lysate (whole cell)
ab94057 is a 293T cell transfected lysate in which Human Prolactin has been transiently over-expressed using a pCMV-Prolactin plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details on how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundVarious hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands.
Our Abpromise guarantee covers the use of ab94057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94057 at 15 µg/lane on an SDS-PAGE gel
All lanes : Anti-Prolactin/PRL antibody (ab57741) at 1/500 dilution
Lane 1 :
Prolactin/PRL overexpression 293T lysate (whole cell) (ab94057)
Lane 2 : 293T non-transfected Lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94057 has not yet been referenced specifically in any publications.