Overview

  • Product name

    Anti-Prolyl Endopeptidase antibody
    See all Prolyl Endopeptidase primary antibodies
  • Description

    Rabbit polyclonal to Prolyl Endopeptidase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide within Human Prolyl Endopeptidase aa 25-75. The exact sequence is proprietary.
    Database link: P48147

  • Positive control

    • IP: HEK-293T whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to Prolyl Endopeptidase immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 81 kDa.

Not recommended. ab241984 has not performed satisfactorily when used for WB of Prolyl Endopeptidase in crude preparations (e.g.whole cell lysate). This antibody can be used for WB of enriched (e.g.immunoprecipitated) sources of Prolyl Endopeptidase.

 

Target

Images

  • Prolyl Endopeptidase was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241984 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab241984 at 0.4 µg/mL.

    Lane 1: ab241984 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 3 seconds.

    Lysates prepared in NETN buffer.

     

     

References

ab241984 has not yet been referenced specifically in any publications.

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