Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [3A3E1] to Properdin/PFC
- Suitable for: WB, ELISA
- Reacts with: Mouse, Rat, Human
Product nameAnti-Properdin/PFC antibody [3A3E1]
See all Properdin/PFC primary antibodies
DescriptionMouse monoclonal [3A3E1] to Properdin/PFC
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Mouse, Rat, Human
Full length native protein (purified). This information is considered to be commercially sensitive.
- WB: Human, mouse and rat plasma lysate. Human and mouse serum lysate.
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact email@example.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein A purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab252531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.454 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).|
|ELISA||Use at an assay dependent concentration.
1250 - 5000 ng/ml.
FunctionA positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes.
Involvement in diseaseDefects in CFP are the cause of properdin deficiency (PFD) [MIM:312060]. PFD results in higher susceptibility to bacterial infections; especially to meningococcal infections. Three phenotypes have been reported: complete deficiency (type I), incomplete deficiency (type II), and dysfunction of properdin (type III).
Sequence similaritiesContains 6 TSP type-1 domains.
- Information by UniProt
- BFD antibody
- CFP antibody
- Complement factor P antibody
All lanes : Anti-Properdin/PFC antibody [3A3E1] (ab252531) at 0.454 µg/ml
Lane 1 : Human plasma lysate
Lane 2 : Human serum lysate
Lane 3 : Mouse plasma lysate
Lane 4 : Mouse serum lysate
Lane 5 : Rat plasma lysate
Lysates/proteins at 20 µg per lane.
All lanes : Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 1 second
Blocking/Dilution buffer: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24797388).
ELISA - Anti-Properdin/PFC antibody [3A3E1] (ab252531).
Antigen: Properdin 1000 ng/ml.
ab252531 used at 0-5000 ng/ml.
Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) secondary used at 1/1000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab252531 has been referenced in 1 publication.
- Blatt AZ et al. Properdin-Mediated C5a Production Enhances Stable Binding of Platelets to Granulocytes in Human Whole Blood. J Immunol 196:4671-4680 (2016). PubMed: 27183616