Anti-Properdin/PFC antibody (ab186834)
Key features and details
- Rabbit polyclonal to Properdin/PFC
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Properdin/PFC antibody
See all Properdin/PFC primary antibodies -
Description
Rabbit polyclonal to Properdin/PFC -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human Properdin/PFC aa 1-469.
Database link: P27918 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
1/500 - 1/2000. Predicted molecular weight: 51 kDa.
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/500 - 1/2000. Predicted molecular weight: 51 kDa. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
A positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. -
Involvement in disease
Defects in CFP are the cause of properdin deficiency (PFD) [MIM:312060]. PFD results in higher susceptibility to bacterial infections; especially to meningococcal infections. Three phenotypes have been reported: complete deficiency (type I), incomplete deficiency (type II), and dysfunction of properdin (type III). -
Sequence similarities
Contains 6 TSP type-1 domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5199 Human
- Entrez Gene: 18636 Mouse
- Entrez Gene: 299314 Rat
- Omim: 300383 Human
- SwissProt: P27918 Human
- SwissProt: P11680 Mouse
- Unigene: 53155 Human
- Unigene: 3064 Mouse
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Alternative names
- BFD antibody
- CFP antibody
- Complement factor P antibody
see all
Images
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Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186834. Blue DAPI for nuclear staining.
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All lanes : Anti-Properdin/PFC antibody (ab186834) at 1/500 dilution
Lane 1 : Extracts from 293T cell line
Lane 2 : Extracts from 3T3L1 cell line
Lane 3 : Extracts from mouse liver
Lane 4 : Extracts from mouse brain
Lane 5 : Extracts from mouse kidney
Predicted band size: 51 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab186834 has been referenced in 1 publication.
- Wu Y et al. Properdin Deficiency Impairs Phagocytosis and Enhances Injury at Kidney Repair Phase Post Ischemia-Reperfusion. Front Immunol 12:697760 (2021). PubMed: 34552582