Overview

  • Product name

    Anti-Properdin/PFC antibody
    See all Properdin/PFC primary antibodies
  • Description

    Rabbit polyclonal to Properdin/PFC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Properdin/PFC aa 1-469.
    Database link: P27918

  • Positive control

    • Extracts from 293T, 3T3L1, mouse liver, mouse brain and mouse kidney
  • General notes

     This product was previously labelled as Properdin

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab186834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/2000. Predicted molecular weight: 51 kDa.
IHC-P 1/50 - 1/200.

Target

  • Function

    A positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes.
  • Involvement in disease

    Defects in CFP are the cause of properdin deficiency (PFD) [MIM:312060]. PFD results in higher susceptibility to bacterial infections; especially to meningococcal infections. Three phenotypes have been reported: complete deficiency (type I), incomplete deficiency (type II), and dysfunction of properdin (type III).
  • Sequence similarities

    Contains 6 TSP type-1 domains.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • BFD antibody
    • CFP antibody
    • Complement factor P antibody
    • Complement factor properdin antibody
    • Factor P antibody
    • PFC antibody
    • PFD antibody
    • PROP_HUMAN antibody
    • Properdin antibody
    • Properdin P factor complement antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186834. Blue DAPI for nuclear staining.
  • All lanes : Anti-Properdin/PFC antibody (ab186834) at 1/500 dilution

    Lane 1 : Extracts from 293T cell line
    Lane 2 : Extracts from 3T3L1 cell line
    Lane 3 : Extracts from mouse liver
    Lane 4 : Extracts from mouse brain
    Lane 5 : Extracts from mouse kidney

    Predicted band size: 51 kDa

References

ab186834 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Retina)
Permeabilization
Yes - 0,1% Triton-X-100
Specification
Retina
Blocking step
BSA+Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 04 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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