• Product name
    Propidium Iodide
  • Product overview

    Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized.

    For Microscopy analysis: PI can be viewed using rhodamine(red) filter (?= 536/617). Cells will only be stained if the membrane has been permeated, either naturally (non-viable cells) or with detergents (for fluorescent staining).

    Flow Cytometry analysis: PI staining can be monitored in FL2 (DNA content) or FL3 (viability) channel.

    If used together as control for Annexin V assays ab14082, ab14083 or ab14152, PI should be diluted to 250 µg/ml solution (in PBS) prior use and added as 1 µl/ Annexin V Assay (0.25µg/assay).

    Visit our FAQs page for tips and troubleshooting.

  • Tested applications
    Suitable for: FMmore details



Our Abpromise guarantee covers the use of ab14083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.


  • Vero-DogSLAM (VDS) cells and Vero (African Green Monkey kidney) cells were infected with wtCDV. Cells were fixed, permeabilised and stained with SSPE serum and rabbit anti-human FITC; nuclei were stained using ab14083 propidium iodide. Images were taken using a Nikon Eclipse TE2000-U UV microscope (x400). 


  • Wang et al. used ab14083 to investigate an optimized platform for maintaining proliferation of giant panda mesenchymal stem cells (MSCs). MSCs were fixed in 70% alcohol and 30% PBS at 4°C for 1 hour. Cells are then incubated in the dark with PBS, 20µg/ml propidium iodide (ab14083) and 1% RNaseA for 30 minutes at 37°C. Cell samples are resuspended in PBS and analyzed using FACS Calibur flow cytometry.

    Flow cytometry analysis shows the cells are at different cell cycle stages in different concentrations (0ng/ml and 5ng/ml respectively) of basic fibroblast growth factor (bFGF).



This product has been referenced in:

See all 12 Publications for this product

Customer reviews and Q&As

Here is a link to the PI staining protocol I found, https://www.abcam.com/protocols/flow-cytometric-analysis-of-cell-cycle-with-propidium-iodide-dna-staining

It suggests using a solution of 50ug/mL, so dilute the amount of the 1mg/mL stoc...

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I am very pleased to hear you would like to accept our offer and test these products in flow cytometry. Each code will give you the value listed off your next order before the expiration date. To redeem this offer, please submit an Abreview for flow cy...

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Thank you for your reply.

Both Annexin V-EGFP Apoptosis Detection Reagents (ab14152 and ab14082) contain a protocol booklet in their datasheets. These are the recommended protocols to use along with the Propidium Iodide reagent.


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I confirm the product Propiduim Iodide ab14083 can definitely be used with paraffin-embedded, formalin-fixed tissues.

Thank you for your comprehension, and please do not hesitate to contact us back for further assistance.

Thank you for contacting us.

Yes, Propidium Iodide ab14083 can definitely be used with fixed tissue samples.

Please do not hesitate to contact us if you need any more advice or information.

Vielen Dank für Ihre Geduld.

Wie versprochen habe ich mich nach den aktuellen Details des Propidiumiodid-Staining Protokolls erkundigt – an diesem seit Jahren bewährten Protokoll hat sich nichts geändert. Ein Mitarbeiter ...

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Thank you for sending your data, it was very helpful in this case. Since the first peak is much higher, I suspect it should be a combination of sub G1 and cell debris. Usually if it is cell debris, it should just be at the bottom and there shouldn’t be...

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Thank you for calling Abcam earlier. I am going to email the lab about your query and I just wanted to make sure I had written it down correctly, were you asking: "Will Propidium Iodide label the sub G0 phase" Is that correct? If so, I will go ahead an...

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Thank you for your enquiry and your patience. I do not have any specific advice on why you see staining in the yolk. The best resource is probably the protocols you can find in the zebrafish book at www.zfin.org I believe that methanol washes ma...

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