Recombinant
RabMAb

Recombinant Anti-Prostacyclin receptor antibody [EPR16518] (ab196653)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16518] to Prostacyclin receptor
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Prostacyclin receptor antibody [EPR16518]
    See all Prostacyclin receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR16518] to Prostacyclin receptor

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human Prostacyclin receptor aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: P43119

  • Positive control

    • HepG2 whole cell lysate (ab7900); Human fetal kidney lysate; Human endometrium tissue; HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab196653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 41 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/120.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Receptor for prostacyclin (prostaglandin I2 or PGI2). The activity of this receptor is mediated by G(s) proteins which activate adenylate cyclase.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.

  • Post-translational
    modifications

    Palmitoylation of either Cys-308 or Cys-311 is sufficient to maintain functional coupling to G(s) and signaling.
    Isoprenylation does not influence ligand binding but is required for efficient coupling to the effectors adenylyl cyclase and phospholipase C.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • IP antibody
      • PGI receptor antibody
      • PGI2 receptor antibody
      • PI2R_HUMAN antibody
      • PRIPR antibody
      • Prostacyclin receptor antibody
      • Prostaglandin I2 (prostacyclin) receptor (IP) antibody
      • Prostaglandin I2 receptor antibody
      • Prostanoid IP receptor antibody
      • Ptgir antibody
      see all

    Images

    • Western blot - Anti-Prostacyclin receptor antibody [EPR16518] (ab196653)
      Western blot - Anti-Prostacyclin receptor antibody [EPR16518] (ab196653)
      All lanes : Anti-Prostacyclin receptor antibody [EPR16518] (ab196653) at 1/1000 dilution

      Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
      Lane 2 : Human fetal kidney lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 41 kDa
      Observed band size: 40 kDa
      why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostacyclin receptor antibody [EPR16518] (ab196653)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostacyclin receptor antibody [EPR16518] (ab196653)

      Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling Prostacyclin receptor with ab196653 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cell membrane staining on Human endometrium is observed. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-Prostacyclin receptor antibody [EPR16518] (ab196653)
      Flow Cytometry - Anti-Prostacyclin receptor antibody [EPR16518] (ab196653)

      Flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 (Human liver hepatocellular carcinoma) cells labeling Prostacyclin receptor with ab196653 at 1/120 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

    References

    Publishing research using ab196653? Please let us know so that we can cite the reference in this datasheet.

    ab196653 has not yet been referenced specifically in any publications.

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