Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16518] to Prostacyclin receptor
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Product nameAnti-Prostacyclin receptor antibody [EPR16518]
See all Prostacyclin receptor primary antibodies
DescriptionRabbit monoclonal [EPR16518] to Prostacyclin receptor
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Recombinant fragment within Human Prostacyclin receptor aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: P43119
- HepG2 whole cell lysate (ab7900); Human fetal kidney lysate; Human endometrium tissue; HepG2 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab196653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 41 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionReceptor for prostacyclin (prostaglandin I2 or PGI2). The activity of this receptor is mediated by G(s) proteins which activate adenylate cyclase.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
modificationsPalmitoylation of either Cys-308 or Cys-311 is sufficient to maintain functional coupling to G(s) and signaling.
Isoprenylation does not influence ligand binding but is required for efficient coupling to the effectors adenylyl cyclase and phospholipase C.
Cellular localizationCell membrane.
- Information by UniProt
- IP antibody
- PGI receptor antibody
- PGI2 receptor antibody
All lanes : Anti-Prostacyclin receptor antibody [EPR16518] (ab196653) at 1/1000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 41 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling Prostacyclin receptor with ab196653 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cell membrane staining on Human endometrium is observed. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 (Human liver hepatocellular carcinoma) cells labeling Prostacyclin receptor with ab196653 at 1/120 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
ab196653 has not yet been referenced specifically in any publications.