Recombinant Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP12357] to Prostaglandin D Synthase (Lipocalin)/PDS
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357]
See all Prostaglandin D Synthase (Lipocalin)/PDS primary antibodies -
Description
Rabbit monoclonal [EP12357] to Prostaglandin D Synthase (Lipocalin)/PDS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab188544) -
Positive control
- WB:HepG2, SW480, and THP-1 whole cell lysate IHC-P: Human glioma ICC/IF: HepG2 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP12357 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 21 kDa).Can be blocked with Prostaglandin D Synthase (Lipocalin)/PDS peptide (ab188544).
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IHC-P |
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/200.
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Notes |
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WB
1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 21 kDa).Can be blocked with Prostaglandin D Synthase (Lipocalin)/PDS peptide (ab188544). |
IHC-P
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/200. |
Target
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Function
Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system. -
Tissue specificity
Abundant in the brain and CNS, where it is expressed in tissues of the blood-brain barrier and secreted into the cerebro-spinal fluid. Abundantly expressed in the heart. In the male reproductive system, it is expressed in the testis, epididymis and prostate, and is secreted into the seminal fluid. Expressed in the eye and secreted into the aqueous humor. Lower levels detected in various tissue fluids such as serum, normal urine, ascitic fluid and tear fluid. Also found in a number of other organs including ovary, fimbriae of the fallopian tubes, kidney, leukocytes. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Developmental stage
Expression in the amniotic fluid increases dramatically during weeks 12 to 25 of pregnancy. Levels decrease slowly after 25 weeks. -
Domain
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
Post-translational
modificationsBoth N-glycosylation recognition sites are almost quantitatively occupied by N-glycans of the biantennary complex type, with a considerable proportion of structures bearing a bisecting GlcNAc. Agalacto structure as well as sialylated and nonsialylated oligosaccharides bearing alpha2-3- and/or alpha2-6-linked NeuNAc are present. -
Cellular localization
Rough endoplasmic reticulum. Nucleus membrane. Golgi apparatus. Cytoplasm > perinuclear region. Secreted. Detected on rough endoplasmic reticulum of arachnoid and menigioma cells. Localized to the nuclear envelope, Golgi apparatus, secretory vesicles and spherical cytoplasmic structures in arachnoid trabecular cells, and to circular cytoplasmic structures in meningeal macrophages and perivascular microglial cells. In oligodendrocytes, localized to the rough endoplasmic reticulum and nuclear envelope. In retinal pigment epithelial cells, localized to distinct cytoplasmic domains including the perinuclear region. Also secreted. - Information by UniProt
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Database links
- Entrez Gene: 5730 Human
- Omim: 176803 Human
- SwissProt: P41222 Human
- Unigene: 446429 Human
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Alternative names
- Beta trace protein antibody
- Beta-trace protein antibody
- Cerebrin 28 antibody
see all
Images
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All lanes : Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141) at 1/10000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 15 µg
Lane 2 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Exposure time: 60 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Primary and secondary antibodies: incubated for 1h at room temperature.
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The human glioma paraffin-embedded sections were Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins and labeling ab182141 at 1/200 dilution for 30 mins at room temperature, then followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
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HepG2 cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab182141 at 1/200 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti- Rabbit secondary antibody (ab150081) at 1/1000 dilution at RT for 45 min. ab195889 AlexaFluor® 594-conjugated Anti-alpha Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab182141 overnight at 4° C. Nucleus were visualized using DAPI.
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Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141) at 1/1000 dilution + HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted? -
Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Prostaglandin D Synthase (Lipocalin)/PDS with ab182141 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counterstained with DAPI.
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Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Prostaglandin D Synthase (Lipocalin)/PDS with ab182141 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141) at 1/1000 dilution + Human fetal brain lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 21 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (9)
ab182141 has been referenced in 9 publications.
- Diao B et al. Human kidney is a target for novel severe acute respiratory syndrome coronavirus 2 infection. Nat Commun 12:2506 (2021). PubMed: 33947851
- Xu W et al. Dexamethasone Suppresses Radicular Pain Through Targeting the L-PGDS/PI3K/Akt Pathway in Rats With Lumbar Disc Herniation. Pain Pract 21:64-74 (2021). PubMed: 32640501
- Wu S et al. The prostaglandin synthases, COX-2 and L-PGDS, mediate prostate hyperplasia induced by low-dose bisphenol A. Sci Rep 10:13108 (2020). PubMed: 32753632
- Wegmann R et al. CellSIUS provides sensitive and specific detection of rare cell populations from complex single-cell RNA-seq data. Genome Biol 20:142 (2019). PubMed: 31315641
- Liu YR et al. ER-depletion lowering the 'hypothalamus-uterus-kidney' axis functions by perturbing the renal ERß/Ptgds signalling pathway. Aging (Albany NY) 11:9500-9529 (2019). PubMed: 31708494
- Chai D et al. Multiple sevoflurane exposures during pregnancy inhibit neuronal migration by upregulating prostaglandin D2 synthase. Int J Dev Neurosci 78:77-82 (2019). PubMed: 31499143
- Li X et al. Endogenous ET-1 promotes ANP secretion through activation of COX2-L-PGDS-PPAR? signaling in hypoxic beating rat atria. Peptides 122:170150 (2019). PubMed: 31541683
- Corwin C et al. Prostaglandin D2/J2 signaling pathway in a rat model of neuroinflammation displaying progressive parkinsonian-like pathology: potential novel therapeutic targets. J Neuroinflammation 15:272 (2018). PubMed: 30236122
- Isensee J et al. Subgroup-elimination transcriptomics identifies signaling proteins that define subclasses of TRPV1-positive neurons and a novel paracrine circuit. PLoS One 9:e115731 (2014). PubMed: 25551770