Recombinant
RabMAb

Recombinant Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141)

Overview

  • Product name

    Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357]
    See all Prostaglandin D Synthase (Lipocalin)/PDS primary antibodies
  • Description

    Rabbit monoclonal [EP12357] to Prostaglandin D Synthase (Lipocalin)/PDS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Prostaglandin D Synthase (Lipocalin)/PDS aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: P41222
    (Peptide available as ab188544)

  • Positive control

    • HepG2 whole cell lysate (ab7900); Human fetal brain lysate; Human prostate tissue; HepG2 cells.
  • General notes

     This product was previously labelled as Prostaglandin D Synthase (Lipocalin)

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP12357
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab182141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 21 kDa).Can be blocked with Prostaglandin D Synthase (Lipocalin)/PDS peptide (ab188544).
IHC-P 1/100.
ICC/IF 1/100.

Target

  • Function

    Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system.
  • Tissue specificity

    Abundant in the brain and CNS, where it is expressed in tissues of the blood-brain barrier and secreted into the cerebro-spinal fluid. Abundantly expressed in the heart. In the male reproductive system, it is expressed in the testis, epididymis and prostate, and is secreted into the seminal fluid. Expressed in the eye and secreted into the aqueous humor. Lower levels detected in various tissue fluids such as serum, normal urine, ascitic fluid and tear fluid. Also found in a number of other organs including ovary, fimbriae of the fallopian tubes, kidney, leukocytes.
  • Sequence similarities

    Belongs to the calycin superfamily. Lipocalin family.
  • Developmental stage

    Expression in the amniotic fluid increases dramatically during weeks 12 to 25 of pregnancy. Levels decrease slowly after 25 weeks.
  • Domain

    Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • Post-translational
    modifications

    Both N-glycosylation recognition sites are almost quantitatively occupied by N-glycans of the biantennary complex type, with a considerable proportion of structures bearing a bisecting GlcNAc. Agalacto structure as well as sialylated and nonsialylated oligosaccharides bearing alpha2-3- and/or alpha2-6-linked NeuNAc are present.
  • Cellular localization

    Rough endoplasmic reticulum. Nucleus membrane. Golgi apparatus. Cytoplasm > perinuclear region. Secreted. Detected on rough endoplasmic reticulum of arachnoid and menigioma cells. Localized to the nuclear envelope, Golgi apparatus, secretory vesicles and spherical cytoplasmic structures in arachnoid trabecular cells, and to circular cytoplasmic structures in meningeal macrophages and perivascular microglial cells. In oligodendrocytes, localized to the rough endoplasmic reticulum and nuclear envelope. In retinal pigment epithelial cells, localized to distinct cytoplasmic domains including the perinuclear region. Also secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta trace protein antibody
    • Beta-trace protein antibody
    • Cerebrin 28 antibody
    • Cerebrin-28 antibody
    • glutathione independent PGD synthase antibody
    • Glutathione independent PGD synthetase antibody
    • Glutathione-independent PGD synthase antibody
    • L PGDS antibody
    • Lipocalin type prostaglandin D synthase antibody
    • Lipocalin-type prostaglandin-D synthase antibody
    • LPGDS antibody
    • PDS antibody
    • PGD2 antibody
    • PGD2 synthase antibody
    • PGDS antibody
    • PGDS2 antibody
    • prostaglandin D synthase antibody
    • prostaglandin D2 synthase (21 kDa, brain) antibody
    • prostaglandin D2 synthase (brain) antibody
    • prostaglandin D2 synthase 21kDa (brain) antibody
    • Prostaglandin D2 synthase antibody
    • prostaglandin H2 D isomerase antibody
    • Prostaglandin-D2 synthase antibody
    • Prostaglandin-H2 D-isomerase antibody
    • PTGDS antibody
    • PTGDS_HUMAN antibody
    • RP23-47P18.11-006 antibody
    see all

Images

  • Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141) at 1/1000 dilution + HepG2 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?

  • Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] (ab182141) at 1/1000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 21 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Prostaglandin D Synthase (Lipocalin)/PDS with ab182141 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counterstained with DAPI.

  • Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Prostaglandin D Synthase (Lipocalin)/PDS with ab182141 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Wegmann R  et al. CellSIUS provides sensitive and specific detection of rare cell populations from complex single-cell RNA-seq data. Genome Biol 20:142 (2019). Read more (PubMed: 31315641) »
  • Corwin C  et al. Prostaglandin D2/J2 signaling pathway in a rat model of neuroinflammation displaying progressive parkinsonian-like pathology: potential novel therapeutic targets. J Neuroinflammation 15:272 (2018). Read more (PubMed: 30236122) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

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Verified customer

Submitted Jun 30 2017

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