Recombinant
RabMAb

Recombinant Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] - BSA and Azide free (ab236119)

Overview

  • Product name

    Anti-Prostaglandin D Synthase (Lipocalin)/PDS antibody [EP12357] - BSA and Azide free
    See all Prostaglandin D Synthase (Lipocalin)/PDS primary antibodies
  • Description

    Rabbit monoclonal [EP12357] to Prostaglandin D Synthase (Lipocalin)/PDS - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Prostaglandin D Synthase (Lipocalin)/PDS aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: P41222
    (Peptide available as ab188544)

  • Positive control

    • IHC-P: Human prostate tissue.
  • General notes

    ab236119 is the carrier-free version of ab182141 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab236119 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Prostaglandin D Synthase (Lipocalin)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP12357
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 28 kDa (predicted molecular weight: 21 kDa).Can be blocked with Prostaglandin D Synthase (Lipocalin)/PDS peptide (ab188544).

Target

  • Function

    Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system.
  • Tissue specificity

    Abundant in the brain and CNS, where it is expressed in tissues of the blood-brain barrier and secreted into the cerebro-spinal fluid. Abundantly expressed in the heart. In the male reproductive system, it is expressed in the testis, epididymis and prostate, and is secreted into the seminal fluid. Expressed in the eye and secreted into the aqueous humor. Lower levels detected in various tissue fluids such as serum, normal urine, ascitic fluid and tear fluid. Also found in a number of other organs including ovary, fimbriae of the fallopian tubes, kidney, leukocytes.
  • Sequence similarities

    Belongs to the calycin superfamily. Lipocalin family.
  • Developmental stage

    Expression in the amniotic fluid increases dramatically during weeks 12 to 25 of pregnancy. Levels decrease slowly after 25 weeks.
  • Domain

    Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • Post-translational
    modifications

    Both N-glycosylation recognition sites are almost quantitatively occupied by N-glycans of the biantennary complex type, with a considerable proportion of structures bearing a bisecting GlcNAc. Agalacto structure as well as sialylated and nonsialylated oligosaccharides bearing alpha2-3- and/or alpha2-6-linked NeuNAc are present.
  • Cellular localization

    Rough endoplasmic reticulum. Nucleus membrane. Golgi apparatus. Cytoplasm > perinuclear region. Secreted. Detected on rough endoplasmic reticulum of arachnoid and menigioma cells. Localized to the nuclear envelope, Golgi apparatus, secretory vesicles and spherical cytoplasmic structures in arachnoid trabecular cells, and to circular cytoplasmic structures in meningeal macrophages and perivascular microglial cells. In oligodendrocytes, localized to the rough endoplasmic reticulum and nuclear envelope. In retinal pigment epithelial cells, localized to distinct cytoplasmic domains including the perinuclear region. Also secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta trace protein antibody
    • Beta-trace protein antibody
    • Cerebrin 28 antibody
    • Cerebrin-28 antibody
    • glutathione independent PGD synthase antibody
    • Glutathione independent PGD synthetase antibody
    • Glutathione-independent PGD synthase antibody
    • L PGDS antibody
    • Lipocalin type prostaglandin D synthase antibody
    • Lipocalin-type prostaglandin-D synthase antibody
    • LPGDS antibody
    • PDS antibody
    • PGD2 antibody
    • PGD2 synthase antibody
    • PGDS antibody
    • PGDS2 antibody
    • prostaglandin D synthase antibody
    • prostaglandin D2 synthase (21 kDa, brain) antibody
    • prostaglandin D2 synthase (brain) antibody
    • prostaglandin D2 synthase 21kDa (brain) antibody
    • Prostaglandin D2 synthase antibody
    • prostaglandin H2 D isomerase antibody
    • Prostaglandin-D2 synthase antibody
    • Prostaglandin-H2 D-isomerase antibody
    • PTGDS antibody
    • PTGDS_HUMAN antibody
    • RP23-47P18.11-006 antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Prostaglandin D Synthase (Lipocalin)/PDS with ab182141 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counterstained with DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182141).

  • Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Prostaglandin D Synthase (Lipocalin)/PDS with ab182141 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182141).

References

ab236119 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab236119.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up