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Rabbit polyclonal to Prostaglandin E Receptor EP2
Predicted to work with:
Rat, Sheep, Horse, Cow
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Prostaglandin E Receptor EP2.
(Peptide available as
This antibody gave a positive signal in Human Placenta Tissue Lysate and JEG-3 Whole Cell Lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 40 kDa).
Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.
Placenta and lung.
Belongs to the G-protein coupled receptor 1 family.
Information by UniProt
PGE receptor EP2 subtype antibody
Western blot - Anti-Prostaglandin E Receptor EP2 antibody (ab73191)
All lanes :
Anti-Prostaglandin E Receptor EP2 antibody (ab73191) at 1 µg/ml
Lane 1 :
Human placenta tissue lysate - total protein (
Lane 2 :
JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Secondary All lanes :
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (
) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
42 kDa (
why is the actual band size different from the predicted?
Prostaglandin E Receptor EP2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"