The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 - 7 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.
Placenta and lung.
Belongs to the G-protein coupled receptor 1 family.