Overview

  • Product name

    Anti-Prostaglandin E Receptor EP2/PTGER2 antibody
    See all Prostaglandin E Receptor EP2/PTGER2 primary antibodies
  • Description

    Rabbit polyclonal to Prostaglandin E Receptor EP2/PTGER2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human Prostaglandin E Receptor EP2/PTGER2 (C terminal).

  • Positive control

    • Mouse liver cell extract, Human Uterus, Myometrium tissue
  • General notes

     This product was previously labelled as Prostaglandin E Receptor EP2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab124419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 40 kDa.
ELISA 1/1000.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.
  • Tissue specificity

    Placenta and lung.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • EP2 antibody
    • PE2R2_HUMAN antibody
    • PGE receptor EP2 subtype antibody
    • PGE2 receptor EP2 subtype antibody
    • Prostaglandin E receptor 2 EP2 subtype antibody
    • Prostaglandin E receptor 2 subtype EP2 53kDa antibody
    • Prostaglandin E receptor 2 subtype EP2 antibody
    • Prostaglandin E2 receptor antibody
    • Prostaglandin E2 receptor EP2 subtype antibody
    • Prostanoid EP2 receptor antibody
    • Ptger2 antibody
    see all

Images

  • All lanes : Anti-Prostaglandin E Receptor EP2/PTGER2 antibody (ab124419) at 1/500 dilution

    Lane 1 : Mouse liver cell extract
    Lane 2 : Mouse liver cell extract with synthesized peptide

    Predicted band size: 40 kDa

  • Staining of Prostaglandin E Receptor EP2/PTGER2 in Paraffin-embedded Human Uterus, Myometrium tissue by Immunohistochemistry using ab124419 at a concentration of 5 µg/ml.

References

This product has been referenced in:

  • Mochocki M  et al. Expression of prostaglandin E2 prostanoid receptor EP2 and interleukin-1ß in laryngeal carcinoma - preliminary study. Contemp Oncol (Pozn) 19:113-9 (2015). IHC-P ; Human . Read more (PubMed: 26034388) »
  • Kaul V  et al. Prostanoid receptor 2 signaling protects T helper 2 cells from BALB/c mice against activation-induced cell death. J Biol Chem 287:25434-9 (2012). WB ; Mouse . Read more (PubMed: 22654101) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your inquiry.

The lab that tested this antibody and produced the image (human formalin-fixed paraffin-embedded tissues)on the datasheet as result used the following antigen retrieval method:

1.) Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes

2.) Remove from heat and let stand at room temperature in buffer - 20 minutes

3.) Rinse in 1X TBS with Tween (TBST) – 1 minute @ RT

I hope this information is helpful and wish you good luck with your research.

Read More

Answer

To our knowledge, the antibody ab124419 has not been published, but the in-house western blot and IHC data are very clean.

Read More

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