Product nameAnti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (HRP)
See all Prostaglandin E Receptor EP2/PTGER2 primary antibodies
DescriptionRabbit monoclonal [EPR8030(B)] to Prostaglandin E Receptor EP2/PTGER2 (HRP)
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide within Human Prostaglandin E Receptor EP2/PTGER2 (C terminal). The exact sequence is proprietary.
Database link: P43116
- WB: Placenta (Human), Lung (Human), JAR (Human Choriocarcinoma), WI38 (Human lung fibroblast cell line) and Liver (Mouse) Whole Cell Lysates. IHC-P: FFPE normal human placenta tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.1% Proclin
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (ab167171)
- Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] - BSA and Azide free (ab236779)
- Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (Alexa Fluor® 488) (ab246777)
- Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (Alexa Fluor® 647) (ab246778)
Our Abpromise guarantee covers the use of ab203462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 40 kDa).|
FunctionReceptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.
Tissue specificityPlacenta and lung.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- EP2 antibody
- PE2R2_HUMAN antibody
- PGE receptor EP2 subtype antibody
All lanes : Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (HRP) (ab203462) at 1/5000 dilution
Lane 1 : Placenta (Human) Tissue Lysate - adult normal tissue
Lane 2 : Lung (Human) Whole Cell Lysate - fetal normal tissue
Lane 3 : JAR (Human Choriocarcinoma) Whole Cell Lysate
Lane 4 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate
Lane 5 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab203462 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
IHC image of Prostaglandin E Receptor EP2/PTGER2 staining in a section of formalin-fixed paraffin-embedded normal human placenta*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab203462, 1/1000 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
ab203462 has not yet been referenced specifically in any publications.