Recombinant
RabMAb

Recombinant Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (HRP) (ab203462)

Overview

  • Product name

    Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (HRP)
    See all Prostaglandin E Receptor EP2/PTGER2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8030(B)] to Prostaglandin E Receptor EP2/PTGER2 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human Prostaglandin E Receptor EP2/PTGER2 (C terminal). The exact sequence is proprietary.
    Database link: P43116

  • Positive control

    • WB: Placenta (Human), Lung (Human), JAR (Human Choriocarcinoma), WI38 (Human lung fibroblast cell line) and Liver (Mouse) Whole Cell Lysates. IHC-P: FFPE normal human placenta tissue.
  • General notes

     This product was previously labelled as Prostaglandin E Receptor EP2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab203462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 55 kDa (predicted molecular weight: 40 kDa).

Target

  • Function

    Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.
  • Tissue specificity

    Placenta and lung.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • EP2 antibody
    • PE2R2_HUMAN antibody
    • PGE receptor EP2 subtype antibody
    • PGE2 receptor EP2 subtype antibody
    • Prostaglandin E receptor 2 EP2 subtype antibody
    • Prostaglandin E receptor 2 subtype EP2 53kDa antibody
    • Prostaglandin E receptor 2 subtype EP2 antibody
    • Prostaglandin E2 receptor antibody
    • Prostaglandin E2 receptor EP2 subtype antibody
    • Prostanoid EP2 receptor antibody
    • Ptger2 antibody
    see all

Images

  • All lanes : Anti-Prostaglandin E Receptor EP2/PTGER2 antibody [EPR8030(B)] (HRP) (ab203462) at 1/5000 dilution

    Lane 1 : Placenta (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Lung (Human) Whole Cell Lysate - fetal normal tissue
    Lane 3 : JAR (Human Choriocarcinoma) Whole Cell Lysate
    Lane 4 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate
    Lane 5 : Liver (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab203462 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • IHC image of Prostaglandin E Receptor EP2/PTGER2 staining in a section of formalin-fixed paraffin-embedded normal human placenta*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab203462, 1/1000 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References

ab203462 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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