Anti-Prostaglandin E Receptor EP4 antibody (ab45295)


  • Product name
    Anti-Prostaglandin E Receptor EP4 antibody
    See all Prostaglandin E Receptor EP4 primary antibodies
  • Description
    Rabbit polyclonal to Prostaglandin E Receptor EP4
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to Human Prostaglandin E Receptor EP4 aa 1-100 conjugated to keyhole limpet haemocyanin.
    Database link: P35408
    (Peptide available as ab45294)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Raji and Ramos



Our Abpromise guarantee covers the use of ab45295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 53 kDa).


  • Function
    Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.
  • Tissue specificity
    High in intestine and in peripheral blood mononuclear cells; low in lung, kidney, thymus, uterus, vasculature and brain. Not found in liver, heart, retina oe skeletal muscle.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EP 4 antibody
    • EP4 antibody
    • EP4R antibody
    • MGC126583 antibody
    • PE2R4_HUMAN antibody
    • PGE receptor EP4 subtype antibody
    • PGE2 receptor EP4 subtype antibody
    • Prostaglandin E receptor 4 antibody
    • Prostaglandin E receptor 4 subtype EP4 antibody
    • Prostaglandin E2 receptor antibody
    • Prostaglandin E2 receptor EP4 subtype antibody
    • Prostanoid EP4 receptor antibody
    • PTGER 2 antibody
    • PTGER 4 antibody
    • PTGER2 antibody
    • PTGER4 antibody
    see all


  • ICC/IF image of ab45295 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45295, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (5 min) HeLa cells at 5µg/ml.
  • Anti-Prostaglandin E Receptor EP4 antibody (ab45295) at 1 µg/ml + Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 20 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 73 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 20 minutes

    ab45295 detects a 73 kDa band in Western Blot which we believe corresponds to the glycosylated form of the protein.


This product has been referenced in:
  • Wang J  et al. Activation of PGE2/EP2 and PGE2/EP4 signaling pathways positively regulate the level of PD-1 in infiltrating CD8+T cells in patients with lung cancer. Oncol Lett 15:552-558 (2018). Read more (PubMed: 29285200) »

See 1 Publication for this product

Customer reviews and Q&As

Western blot
Human Tissue lysate - whole (Abdominal endometriotic lesion)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
15 µg
Abdominal endometriotic lesion
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 22 2017

Thank you for your reply.

You would probably be best suited using a kit that is able to isolate the membrane fraction completely if you are looking at a membrane protein like Prostaglandin E Receptor EP4. You may want to use a kit like our ab...

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Thank you for this feedback! I am happy to hear that it now works :-)

By the way, if you like you could submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this vial of antibody.

The details you have kindly provided will provide us with vital...

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For a Jurkat cell lysate for western blotting, I recommend item ab7899:

Click here (or use the following:

The lysate is presented in SDS-PAGE loading buffer:

50 mM Tris-...

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Thank you for contacting us. Your customer can use a Jurkat cell lysate as a positive control. We have the lysate as ab7899: Click here (or use the following: The Jurkat cell lysate is also an appropria...

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