Anti-Prostaglandin E Synthase/MPGES-1 antibody (ab62050)
- Datasheet
- References (6)
- Protocols
Overview
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Product name
Anti-Prostaglandin E Synthase/MPGES-1 antibody
See all Prostaglandin E Synthase/MPGES-1 primary antibodies -
Description
Rabbit polyclonal to Prostaglandin E Synthase/MPGES-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Cow, Dog, Pig, Xenopus laevis, Monkey, Zebrafish
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Immunogen
Synthetic peptide corresponding to Human Prostaglandin E Synthase/MPGES-1 (N terminal) conjugated to keyhole limpet haemocyanin.
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Positive control
- Human testis tissue.
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General notes
Previously labelled as Prostaglandin E Synthase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab62050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 10 µg/ml. |
Target
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Function
Catalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). -
Sequence similarities
Belongs to the MAPEG family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 417193 Chicken
- Entrez Gene: 282019 Cow
- Entrez Gene: 9536 Human
- Entrez Gene: 64292 Mouse
- Entrez Gene: 59103 Rat
- Entrez Gene: 544666 Zebrafish
- Omim: 605172 Human
- SwissProt: O14684 Human
see all -
Alternative names
- Glutathione S transferase 1 like 1 antibody
- MGC10317 antibody
- MGST IV antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostaglandin E Synthase/MPGES-1 antibody (ab62050)This image is courtesy of an anonymous Abreviewab62050 staining Prostaglandin E Synthase/MPGES-1 in Mouse testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton-X and blocked with 10% serum for 30 minutes at 24°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50) for 20 hours at 4°C. ab150075, a donkey anti-rabbit Alexa Fluor® 647- (1/200) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostaglandin E Synthase/MPGES-1 antibody (ab62050)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue, staining Prostaglandin E Synthase/MPGES-1 with ab62050 at 10 µg/ml.
References
This product has been referenced in:
- Chen Z et al. CRISPR/Cas9-based liver-derived reporter cells for screening of mPGES-1 inhibitors. J Enzyme Inhib Med Chem 34:799-807 (2019). Read more (PubMed: 30879343) »
- Berisha B et al. Prostaglandins in Superovulation Induced Bovine Follicles During the Preovulatory Period and Early Corpus Luteum. Front Endocrinol (Lausanne) 10:467 (2019). Read more (PubMed: 31354631) »
