Western blot abreview for Anti-Prostaglandin E Synthase / MPGES-1 antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (A549 cells)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
20 µg
Specification
A549 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% Marvel TBS-T
Dilution
1/500

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: anti-rabbit HRP linked
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
8 minute(s) and 0 second(s)
Bands
Specific: 19 kDa

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Submitted Jun 10 2010

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