Recombinant Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] (ab180589)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13765] to Prostaglandin E Synthase/MPGES-1
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765]
See all Prostaglandin E Synthase/MPGES-1 primary antibodies -
Description
Rabbit monoclonal [EPR13765] to Prostaglandin E Synthase/MPGES-1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- NIH 3T3, Jurkat and A549 cell line lysates; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 0.05% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13765 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 13 kDa (predicted molecular weight: 17 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 13 kDa (predicted molecular weight: 17 kDa). |
Target
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Function
Catalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). -
Sequence similarities
Belongs to the MAPEG family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 9536 Human
- Entrez Gene: 64292 Mouse
- Entrez Gene: 59103 Rat
- Omim: 605172 Human
- SwissProt: O14684 Human
- SwissProt: Q9JM51 Mouse
- Unigene: 146688 Human
- Unigene: 28768 Mouse
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Alternative names
- Glutathione S transferase 1 like 1 antibody
- MGC10317 antibody
- MGST IV antibody
see all
Images
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All lanes : Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] (ab180589) at 1/1000 dilution
Lane 1 : Lysate of NIH 3T3 cells with Immunizing peptide
Lane 2 : Lysate of NIH 3T3 cells
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 17 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted? -
All lanes : Anti-Prostaglandin E Synthase/MPGES-1 antibody [EPR13765] (ab180589) at 1/1000 dilution
Lane 1 : NIH 3T3 cell line lysate
Lane 2 : Jurkat cell line lysate
Lane 3 : HeLa cell line lysate
Lane 4 : A549 cell line lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 17 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (7)
ab180589 has been referenced in 7 publications.
- Kojima F et al. Facilitation of colonic T cell immune responses is associated with an exacerbation of dextran sodium sulfate-induced colitis in mice lacking microsomal prostaglandin E synthase-1. Inflamm Regen 42:1 (2022). PubMed: 34983695
- Hagiwara M et al. MUC1-C integrates type II interferon and chromatin remodeling pathways in immunosuppression of prostate cancer. Oncoimmunology 11:2029298 (2022). PubMed: 35127252
- Miek L et al. Staphylococcus aureus controls eicosanoid and specialized pro-resolving mediator production via lipoteichoic acid. Immunology 166:47-67 (2022). PubMed: 35143048
- Monteleone NJ & Lutz CS miR-708 Negatively Regulates TNFa/IL-1ß Signaling by Suppressing NF-?B and Arachidonic Acid Pathways. Mediators Inflamm 2021:5595520 (2021). PubMed: 33776573
- Monteleone NJ & Lutz CS miR-708-5p targets oncogenic prostaglandin E2 production to suppress a pro-tumorigenic phenotype in lung cancer cells. Oncotarget 11:2464-2483 (2020). PubMed: 32655834
- Monteleone NJ & Lutz CS miR-708-5p enhances erlotinib/paclitaxel efficacy and overcomes chemoresistance in lung cancer cells. Oncotarget 11:4699-4721 (2020). PubMed: 33473256
- Bogdan D et al. Fatty acid-binding protein 5 controls microsomal prostaglandin E synthase 1 (mPGES-1) induction during inflammation. J Biol Chem 293:5295-5306 (2018). PubMed: 29440395