Overview

  • Product name

    Prostaglandin E1 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Low 24 53pg/ml 4.6%
    Medium 24 246pg/ml 9.5%
    High 24 1103pg/ml 13.7%
    Inter-assay
    Sample n Mean SD CV%
    Low 8 49pg/ml 9.3%
    Medium 8 214pg/ml 11%
    High 8 737pg/ml 6.2%
  • Sample type

    Cell culture supernatant, Saliva, Urine, Serum, Plasma, Tissue Culture Media
  • Assay type

    Competitive
  • Sensitivity

    5.58 pg/ml
  • Range

    4.88 pg/ml - 5000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Saliva 107.2 % - %
    Urine 109.9 % - %
    Serum 87 % - %
    Plasma 107.7 % - %
    Tissue Culture Media 90% - 110%

  • Assay time

    3h 0m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
    Predicted to work with: a wide range of other species
  • Product overview

    Abcam’s Prostaglandin E1 in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prostaglandin E1 in, plasma, serum, urine, tissue culture media and other biological fluids.

    A donkey anti-sheep IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-PGE1 antigen and a polyclonal sheep antibody specific to PGE1. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the Alkaline Phosphatase enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of PGE1 captured in the plate.

  • Notes

    Prostaglandin E1 (PGE1) is synthesized from dihomo-γ-linolenic acid (DGLA). PGE1 has been shown to have a number of biological actions, including vasodilation, proliferation of vascular smooth muscle cells, platelet aggregation and has been shown to have insulin-like actions. Its effects are induced by receptor mediated elevation of cAMP. PGE1 is the major prostaglandin in semen.

    Cross Reactivity

    CompoundCross Reactivity
     PGE1100%
     PGE26.50%
     PGE32.22%
     13,14-dihydro-PGE11.50%
     PGE01.45%
     15-keto-PGE11.15%
     13,14-dihydro-15-keto-PGE10.19%
     PGF0.14%
     PGF0.04%
     6-keto-PGF<0.1%
     PGA2<0.1%
     PGD2<0.1%
     PGB1<0.1%
     13,14-dihydro-15-keto-PGF<0.1%
     6,15-keto-13,14-dihydro-PGF<0.1%
     Thromboxane B2<0.1%
     Misoprostol<0.1%
     2-Arachidonoylglycerol<0.1%
     Anandamide<0.1%
  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab133024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab133024

Protocols

References

ab133024 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Competitive, accurate PGE1 ELISA

Excellent Good 4/5 (Ease of Use)
Abreviews
The ELISA is easy to use, however certain samples need an acid treatment which prolongs and complicates the assay. I had cell supernatants which were assayed untreated but were diluted with the culture medium. It's a competitive ELISA and the higher the O.D. the less PGE1 is present in the sample. The calculation of values is a bit oddly explained in the product booklet, but actually fairly easy. Overall good ELISA.

Abcam user community

Verified customer

Submitted Jan 31 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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