Overview

  • Product name
    Anti-Prostaglandin E2 antibody
    See all Prostaglandin E2 primary antibodies
  • Description
    Rabbit polyclonal to Prostaglandin E2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Highly pure Prostaglandin E2 conjugated to bovine thyroglobulin

  • Positive control
    • Purified PGE2

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium azide
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000 - 1/100000.
IHC-P Use at an assay dependent concentration.
ICC Use at an assay dependent concentration. PubMed: 23762835

Target

  • Relevance
    Prostaglandin E2 (PGE2) is an extensively studied prostaglandin owing to its predominance in inflammation, cancer, atherosclerosis, autoimmune disease, and sepsis.
  • Alternative names
    • PG E2 antibody
    • PGE 2 antibody
    • PGE2 antibody
    • ProstaglandinE2 antibody

References

This product has been referenced in:
  • Li J  et al. Methyl Salicylate Lactoside Protects Neurons Ameliorating Cognitive Disorder Through Inhibiting Amyloid Beta-Induced Neuroinflammatory Response in Alzheimer's Disease. Front Aging Neurosci 10:85 (2018). Read more (PubMed: 29636677) »
  • Ho ATV  et al. Prostaglandin E2 is essential for efficacious skeletal muscle stem-cell function, augmenting regeneration and strength. Proc Natl Acad Sci U S A 114:6675-6684 (2017). IHC ; Mouse . Read more (PubMed: 28607093) »
See all 12 Publications for this product

Customer reviews and Q&As

1-10 of 17 Abreviews or Q&A

Answer

Thank you very much for your interest in ab2318 Anti-Prostaglandin E2 antibody.

To our knowledge, ab2318 has still not been tested in a cell culture assay due to the sodium azide. As my collegue suggest it is possible to extract the chemical via a dialysis step to purify the antibody or to use our purification kit, whichwouldpurify this whole serum antibody on top of this. The purification kit can be found here:

https://www.abcam.com/index.html?datasheet=109208 (or use the following: https://www.abcam.com/index.html?datasheet=109208).

As the application hasn't been tested, I can offer a discount off a future purchase if you buy ab2318 now, test it in cell culture assay and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab2318 in cell culture assay. I will then send a discount code. This code must be issued before purchasing ab2318 so please wait for my reply before ordering.

2. Purchase ab2318 either by phone, fax, or online (www.abcam.com).

3. Test it in cell culture assay.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab2318 turns out to be unsuitable for cell culture assay, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: (10 mM Sodium citrate, 0.05% Tween 20, pH 6.0)
Permeabilization
Yes - 0.5% Tween 20
Specification
spleen
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 27 2016

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (10 % SDS polyacylamide gel)
Sample
Dog Cell lysate - whole cell (adipose tissue derived mesenchymal stem cell)
Specification
adipose tissue derived mesenchymal stem cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Seunghoon Lee

Verified customer

Submitted Apr 09 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Sample
Mouse Tissue sections (mouse skin)
Specification
mouse skin
Permeabilization
Yes - Tween-20
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 22 2014

Question
Answer

Unfortunately, we do not have information regarding cross reactivity of ab2318 to a PEG2 metabolite like 13,14-dihydro-15-keto PGE2.

Read More

Answer

DISCOUNT CODE: XXXXXXX
Expiration date: 20-05-2013

I am very pleased to hear you would like to accept our offer and test ab2318 in IP. This code will give you 100% off your next order before the expiration date. To redeem this offer, please submit an Abreview for IP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

Read More

Answer

Thank you for contacting us and your interest in our products.

We unfortunately do not have the Anti-Prostaglandin E2 antibody (ab2318) available without sodium azide present.

We do however have purification kits available from our catalogue which can be used to remove the azide. These can be viewed from the following links:

https://www.abcam.com/index.html?pageconfig=resource&rid=13489

I hopethis information has been of help. If you have any further questions, please do not hesitate to contact us again.

Read More

Answer

Thank you for your inquiry.

I have to confirm thatab2318 is not eligible for the testing programmewith different species.

The target Prostaglandin E2 is not a protein but a lipid. This chemical will be the same independently of the species.

For more information on the structure please follow this link:

http://en.wikipedia.org/wiki/Prostaglandin_E2


Your customer should not have any problems detecting Prostaglandin E2 in rabbit samples.

Please keep in mind that since PGE2 is a lipid, extraction buffers containing detergents (for preparing tissue samples) are not recommended. Mechanical disruption in a buffer such as PBS is recommended instead. For serum and plasma, simple dilution should be sufficient.

I hope this information is helpful and wish your customers good luck for their experiments.

Read More

Answer

Thank you for contacting us.


I performed a protein alignment with the target immunogens and the rabbit protein sequences for the antibodies. We recommend that alignment should be over 85% to predict that an antibody will detect in a different species.

Here are the results, similarities are in %:

ab47027 Anti-CGRP antibody: 85%

ab14184 Anti-Substance P antibody [SP-DE4-21]: 100%

ab106291 Anti-Substance P antibody: 100%

ab2318: As mentioned in the Scientific Support section, PGE2 is a lipid rather than a protein and is therefore not species specific. There should not be any particular problems using this assay to detect PGE2 from rabbit. Optimization for different sample types will have to be done by the end user. Please keep in mind that since PGE2 is a lipid, extraction buffers containing detergents (for preparing tissue samples) are not recommended. Mechanical disruption in a buffer such as PBS is recommended instead. For serum and plasma, simple dilution should be sufficient.


I can offer a discount off a future purchase if your customer buys one or more of the antibodiesnow, test it in rabbit and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback.


If they are interested in this offer, please let me know.

Read More

Answer

DISCOUNT CODE: XXXXX Expiration date: 12/05/2012 I am very pleased to hear you would like to accept our offer and testab2318 in Rat in IHC-P. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forRat in IHC-Pand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up