Overview

  • Product name

    Prostaglandin E2 ELISA Kit
    See all PGE2 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Low PGE2 24 116pg/ml 8.9%
    Medium PGE2 24 492pg/ml 5.8%
    High PGE2 24 2416pg/ml 17.5%
    Inter-assay
    Sample n Mean SD CV%
    Low PGE2 8 111pg/ml 3%
    Medium PGE2 8 419pg/ml 5.1%
    High PGE2 8 1902pg/ml 3.9%
  • Sample type

    Cell culture supernatant, Saliva, Urine, Serum, Plasma, Tissue, Whole Blood, Cell Lysate
  • Assay type

    Competitive
  • Sensitivity

    13.4 pg/ml
  • Range

    39.1 pg/ml - 2500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Saliva 123.3 % - %
    Urine 108.9 % - %
    Whole Blood 101.2 % - %
    Tissue Culture Media 104.4 % - %

  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Species independent
  • Product overview

    PGE2 ELISA kit (Prostaglandin E2) is a competitive ELISA designed for the accurate quantitative measurement of Prostaglandin E2 in serum, saliva, urine and tissue culture media and other biological fluids.


    A mouse IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Prostaglandin E2 antibody. After incubation the excess reagents are washed away and pNpp substrate is added and is catalyzed by AP to produce a yellow color. The intensity of the yellow coloration is inversely proportional to the amount of Prostaglandin E2 captured in the plate.


    This kit is unsuitable for mouse serum as samples containing mouse IgG may interfere with the assay.


    Tissue/Cell lysate have not been internaly validated so we do not have specific protocols for them however a number of users have had successful results using these sample types.

  • Notes

    Prostaglandin E2 (PGE2) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGE2 has been shown to have a number of biological actions, including vasodilation, both anti- and proinflammatory action, modulation of sleep/wake cycles, and facilitation of the replication of human immunodeficiency virus. It elevates cAMP levels, stimulates bone resorption, and has thermoregulatory effects. It has been shown to be a regulator of sodium excretion and renal hemodynamics.

    Cross Reactivity

    Compound % Cross Reactivity
    PGE2 100
    PGE1 70
    PGE3 16.3
    PGF 1.4
    PGF 0.7
    6-keto-PGF 0.6
    PGA2 0.1
    PGB1 0.1
    13,14-dihydro-15-keto-PGF <0.1
    6,15-keto-13,14-dihydro-PGF <0.1
    Thromboxane B2 <0.1
    2-Arachidonoylglycerol <0.1
    Anandamide <0.1
    PGD2 <0.1
    Arachadonic Acid <0.1
  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab133021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Prostaglandin E2 measured in biological fluids and cell culture supernatants showing quantity (pg) per mL of tested sample. Samples were diluted 1-9 fold.

Protocols

References

This product has been referenced in:

  • Fei J  et al. Luteolin inhibits IL-1ß-induced in?ammation in rat chondrocytes and attenuates osteoarthritis progression in a rat model. Biomed Pharmacother 109:1586-1592 (2019). Read more (PubMed: 30551412) »
  • Shan X  et al. Edoxaban improves atrial fibrillation and thromboembolism through regulation of the Wnt-ß-induced PI3K/ATK-activated protein C system. Exp Ther Med 17:3509-3517 (2019). Read more (PubMed: 30988731) »
See all 26 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Answer

The correction at 570-590nm was included to correct for any imperfections in the plastic of the plate. However, these days the plates are made with such a great quality by the plate manufacturers that this correction is not needed. It would not hurt to do it but we actually just read all of our ELISAs at their detection wavelength of 405 or 450nm depending on the assay. Therefore you can just read ab133021 at 405nm if you would like. If you decide to do the correction then you should be able to indicate that in a second read on a plate reader and the software should then know to subtract the small OD generated by the correction from the 405nm read. Please address any question on th reading and subtracting to the plate reader manufacturer.


However, since your plate reader can only read at 415nm instead of 405nm you should still be able to see fluorescence. There is only a small difference in wavelength, but the reading will not be as strong as it will at the wavelength we recommend (405nm, which is the optimal wavelength at the peak of the fluorescence).

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GOOD

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Sample: Mouse Cell (Homogenised lung tissue)
Specification: Homogenised lung tissue
Type of Kit Used: ELISA
Positive control: Virally infected lung tissue.
Did you use the Abcam Kit protocol?: Yes
Additional data
Additional Notes: Overall, a good kit.

Abcam user community

Verified customer

Submitted Jan 27 2016

Answer


We would recommend to prepare tissue homogenates using a standard method as the one described below:


​​Dissect the tissue of interest with clean tools, on ice preferably and as quickly as possible to prevent degradation by proteases.
Place the tissue in round bottom microfuge tubes and immerse in liquid nitrogen to "snap freeze". Store samples at -80°C for later use or keep on ice for immediate homogenization.
For a ˜5 mg piece of tissue, add ˜300 µl complete extraction buffer (see cell/tissue extraction buffer recipe below) to the tube and homogenize with an electric homogenizer.
Rinse the blade twice using 300 µl complete extraction buffer for each rinse, then maintain constant agitation for 2 hr at 4°C (e.g. place on an orbital shaker in the cold room).
Centrifuge for 20 min at 13,000 rpm at 4°C. Place on ice, aliquot supernatant (this is the soluble protein extract) to a fresh, chilled tube and store samples at -80°C. Minimize freeze/thaw cycles.




Volumes of lysis buffer must be determined in relation to the amount of tissue present. Typical concentration of final protein extract is >1 mg/ml.

Cell/tissue extraction buffer recipe
100 mM Tris, pH 7.4
150 mM NaCl
1 mM EGTA
1 mM EDTA
1% Triton X-100
0.5% Sodium deoxycholate


General recommendations
Recommended protein extract concentration is at least 1-2 mg/ml.
Typically, serum, plasma, cell and tissue extracts are diluted by 50%.
Prior to use after thawing, centrifuge samples at 10,000 rpm for 5' at 4°C to remove any precipitate.

As explained on page 11 of the protocol booklet, in order to help preserve the PGE2, we suggest the immediate addition of prostaglandin synthetase inhibitors such as indomethacin or meclofenamic acid at concentrations up to 10 μg/mL should be added to either the tissue homogenate or urine and plasma samples.

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Answer

We have published the wash buffer for ab133021 in the catalog as ab176140. A link to the product is given below:

https://www.abcam.com/wash-buffer-concentrate-ab176140.html

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Abreviews
PGE2 human Kit works fine and recognices human PGE2 with no doubts.
It's very easy to use.

As manual says that could recognice mouse samples we've test this possibility with mouse samples and, in our hands, the replica signal is strong enough to be agree with this statment.

See attached picture in which we show standard curve and a simple bar with levels of PGE2 in mouse MSC supenantants.

Abcam user community

Verified customer

Submitted Jul 05 2013

Answer


ab136948 as well as our other PGE2 kits, ab133021, ab133055 and ab136949 are species independent; thus the kit is not specific for any particular species. As long as there enough PGE2 in their rat sample for the kit to detect then the kit should work assuming that the customer has optimized dilution and demonstrated dilutional linearity. ab136948 comes with a goat anti-mouse IgG coated plate so samples containing endogenous mouse IgG’s (such as mouse serum or plasma) may lead to some interference in the assay due to the likelihood for those endogenous mouse IgG’s to bind to the goat anti-mouse IgG antibody on the plate. However, this should not affect rat samples. I have provided a citation below which references using one of our PGE2 kits for rat PGE2 cell lysates.

Microsomal Prostaglandin E Synthase-1 Is a Major Terminal Synthase That Is Selectively Up-Regulated During Cyclooxygenase-2-Dependent Prostaglandin E2 Production in the Rat Adjuvant-Induced Arthritis Model: D. Claveau, et al. ; J. Immunol. 170, 4738 (2003), Application(s): EIA using rat cell lysates, http://www.jimmunol.org/cgi/content/abstract/170/9/4738;

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Answer

Thank you for contacting us.
The following kits can be used to determine the amount of PGE2 in tissue samples however these kits can not be used for extracting the PGE2.
https://www.abcam.com/pge2-elisa-kit-chemiluminescent-ab136948.html
https://www.abcam.com/pge2-elisa-kit-ab133021.html
https://www.abcam.com/pge2-high-sensitivity-elisa-kit-ab133055.html
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.
In order to help preserve the PGE2, we suggest the immediate addition of prostaglandin synthetase inhibitors such as indomethacin or meclofenamic acid at concentrations up to 10 μg/mL should be added to either the tissue homogenate or urine and plasma samples.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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