For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
I am interested in your prostaglandin E2 ELISA kit (ab133021) and still have a few questions towards that:
-The instructions say that the experiment should be measured in a standard microplate reader at 405nm. I only have a reader capable of measuring at 415nm at hand. Is it possible to use the kit with this setting as well?
-the instruction also mention that the measurement should be done with a correction at 570-590nm. What exactly does that mean?
Asked on Jul 15 2016
The correction at 570-590nm was included to correct for any imperfections in the plastic of the plate. However, these days the plates are made with such a great quality by the plate manufacturers that this correction is not needed. It would not hurt to do it but we actually just read all of our ELISAs at their detection wavelength of 405 or 450nm depending on the assay. Therefore you can just read ab133021 at 405nm if you would like. If you decide to do the correction then you should be able to indicate that in a second read on a plate reader and the software should then know to subtract the small OD generated by the correction from the 405nm read. Please address any question on th reading and subtracting to the plate reader manufacturer.
However, since your plate reader can only read at 415nm instead of 405nm you should still be able to see fluorescence. There is only a small difference in wavelength, but the reading will not be as strong as it will at the wavelength we recommend (405nm, which is the optimal wavelength at the peak of the fluorescence).
Anja HoffmannAbcam Scientific Support
Answered on Jul 15 2016