Product nameProstaglandin E2 ELISA Kit (Chemiluminescent)
See all PGE2 kits
Intra-assay Sample n Mean SD CV% PGE2 24 7.07pg/ml = 14.7% PGE2 24 120pg/ml = 4.1% PGE2 24 330pg/ml = 7.4% Inter-assay Sample n Mean SD CV% PGE2 8 59.04pg/ml = 9.2% PGE2 8 182pg/ml = 7.1% PGE2 8 923pg/ml = 5.6%
Sample typeCell culture supernatant, Saliva, Urine, Serum, Plasma, Tissue, Whole Blood, Tissue Culture Media, Male Serum, Female Serum
Sensitivity= 6.03 pg/ml
Range7.81 pg/ml - 1000 pg/ml
Sample specific recovery Sample type Average % Range Saliva = 123.3 % - % Urine = 108.9 % - % Whole Blood = 113.6 % - % Tissue Culture Media = 102.4 % - % Male Serum = 126.1 % - % Female Serum = 113.7 % - %
Assay time3h 00m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human, a wide range of other species
Abcam’s Chemiluminescent Prostaglandin E2 (PGE2) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prostaglandin E2 in biological fluids.
A goat anti-mouse IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Prostaglandin E2 antibody. After incubation the excess reagents are washed away and a chemiluminescent substrate is added, which causes light emission at approximately 530 nm. The intensity of the emitted light is inversely proportional to the amount of Prostaglandin E2 captured in the plate.
Prostaglandin E (PGE ) is formed in a variety of cells from PGH , which itself is synthesized from
arachidonic acid by the enzyme prostaglandin synthetase. PGE has been shown to have a number
of biological actions, including vasodilation, both anti- and proinflammatory action modulation of sleep/wake cycles, and facilitation of the replication of Human immunodeficiency virus. It elevates cAMP levels and stimulates bone resorption, and has thermoregulatory effects. It has been shown to be a regulator of sodium excretion and renal hemodynamics.
Cross Reactivity (%)
1.4 PGF2α 0.7 6-keto-PGF1α 0.6 PGA2 0.1 PGB1 0.1 13,14-dihydro-15-keto-PGF2α <0.1 6,15-keto-13,14-dihydro-PGF1α <0.1 Thromboxane B2 <0.1 2-Arachidonoylglycerol <0.1 Anandamide <0.1 PGD2 <0.1
Tested applicationsSuitable for: Competitive ELISAmore details
Storage instructionsPlease refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 30ml Assay Buffer 1 x 30ml Goat anti-mouse IgG Microplate (12 x 8 wells) 1 x 96 units Lumiphos 530™ CLIA Substrate 1 x 21ml Plate Sealer 1 unit Prostaglandin E2 Standard 1 x 0.5ml Prostaglandin E2 (PGE2) Antibody 1 x 6ml Prostaglandin E2 Alkaline Phosphatase Conjugate 1 x 6ml
RelevanceReceptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.
Cellular localizationCell Membrane
- PG E2
- PGE 2
Our Abpromise guarantee covers the use of ab136948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent concentration.|
ab136948 has not yet been referenced specifically in any publications.