Product nameProstaglandin E2 ELISA Kit (Fluorescent)
See all PGE2 kits
Intra-assay Sample n Mean SD CV% PGE2 24 24.142pg/ml = 21% PGE2 24 16.629pg/ml = 13% PGE2 24 5.392pg/ml = 8.3% Inter-assay Sample n Mean SD CV% PGE2 24.103pg/ml = 11.4% PGE2 15.598pg/ml = 10.7% PGE2 6.574pg/ml = 12.7%
Sample typeCell culture supernatant
Assay typeSandwich (quantitative)
Sensitivity= 684 pg/ml
Range1562 pg/ml - 100000 pg/ml
Sample specific recovery Sample type Average % Range Spike 95% - 108%
Assay durationMultiple steps standard assay
Abcam’s Fluorescent Prostaglandin E2 (PGE2) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prostaglandin E2 in buffers and culture media.
A solution of PGE2 conjugated to fluorescein is added to uncoated wells containing standards or samples. A solution of monoclonal antibody to PGE2 is then added. This binds PGE2 in a competitive manner. This is a Fluorescence polarization immunoassay (FPIA), where the underlying principle is “fluorescence anisotropy” or “fluorescence polarization”. The rate of the rotation of a molecule in motion is indicative of its size. Low amount of analyte results in more antibody molecules bound to the fluorophore probe, read-out: high signal. High amount of analyte results in less antibody molecules bound to the fluorophore probe, read-out: low signal. Hence, the signal is inversely proportional to the concentration of PGE2 in the standards or samples.
The assay should be performed in a black uncoated low-binding microtiter plate (not supplied) and is analyzed using a Fluorescence polarization detector, capable of reading emissions at 520 - 535 nm, with excitation at 485 nm.
Prostaglandin E2 (PGE2) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGE2 has been shown to have a number of biological actions, including vasodilation, both anti- and proinflammatory action, modulation of sleep/wake cycles, and facilitation of the replication of human immunodeficiency virus. It elevates cAMP levels, stimulates bone resorption, and has thermoregulatory effects. It has been shown to be a regulator of sodium excretion and renal hemodynamics.
Compound Cross Reactivity (%)
2.5 6-keto-PGF1α 2.3 PGB2 1.8 PGI2 0.9 TXB2 0.3 Arachidonic acid <0.1 Dihomo-γ-linolenic acid <0.1
Tested applicationsSuitable for: FPIAmore details
Storage instructionsPlease refer to protocols.
Components 1 x 96 tests Assay Buffer 1 Concentrate 1 x 125ml PGE2 FPIA Antibody 1 x 8ml PGE2 FPIA Conjugate Concentrate (fluorescein) 1 x 0.1ml PGE2 Standard 1 x 0.5ml
RelevanceReceptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.
Cellular localizationCell Membrane
- PG E2
- PGE 2
Our Abpromise guarantee covers the use of ab136949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|FPIA||Use at an assay dependent concentration.|
ab136949 has not yet been referenced specifically in any publications.