Overview

  • Product name

    Prostaglandin E2 ELISA Kit (Fluorescent)
    See all PGE2 kits
  • Detection method

    Fluorescent
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    PGE2 24 24.142pg/ml = 21%
    PGE2 24 16.629pg/ml = 13%
    PGE2 24 5.392pg/ml = 8.3%
    Inter-assay
    Sample n Mean SD CV%
    PGE2 24.103pg/ml = 11.4%
    PGE2 15.598pg/ml = 10.7%
    PGE2 6.574pg/ml = 12.7%
  • Sample type

    Cell culture supernatant
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 684 pg/ml
  • Range

    1562 pg/ml - 100000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Spike 95% - 108%

  • Assay duration

    Multiple steps standard assay
  • Product overview

    Abcam’s Fluorescent Prostaglandin E2 (PGE2) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prostaglandin E2 in buffers and culture media.

    A solution of PGE2 conjugated to fluorescein is added to uncoated wells containing standards or samples. A solution of monoclonal antibody to PGE2 is then added. This binds PGE2 in a competitive manner. This is a Fluorescence polarization immunoassay (FPIA), where the underlying principle is “fluorescence anisotropy” or “fluorescence polarization”. The rate of the rotation of a molecule in motion is indicative of its size. Low amount of analyte results in more antibody molecules bound to the fluorophore probe, read-out: high signal. High amount of analyte results in less antibody molecules bound to the fluorophore probe, read-out: low signal. Hence, the signal is inversely proportional to the concentration of PGE2 in the standards or samples.


    The assay should be performed in a black uncoated low-binding microtiter plate (not supplied) and is analyzed using a Fluorescence polarization detector, capable of reading emissions at 520 - 535 nm, with excitation at 485 nm.

  • Notes

    Prostaglandin E2 (PGE2) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGE2 has been shown to have a number of biological actions, including vasodilation, both anti- and proinflammatory action, modulation of sleep/wake cycles, and facilitation of the replication of human immunodeficiency virus. It elevates cAMP levels, stimulates bone resorption, and has thermoregulatory effects. It has been shown to be a regulator of sodium excretion and renal hemodynamics.

     

    Compound Cross Reactivity (%)

    PGE2

    100

    PGE1

    100

    PGD2

    11

    PGF2α

    2.5
    6-keto-PGF1α 2.3
    PGB2 1.8
    PGI2 0.9
    TXB2 0.3
    Arachidonic acid <0.1
    Dihomo-γ-linolenic acid <0.1
  • Tested applications

    Suitable for: FPIAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab136949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FPIA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab136949.

Protocols

References

ab136949 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer


ab136948 as well as our other PGE2 kits, ab133021, ab133055 and ab136949 are species independent; thus the kit is not specific for any particular species. As long as there enough PGE2 in their rat sample for the kit to detect then the kit should work assuming that the customer has optimized dilution and demonstrated dilutional linearity. ab136948 comes with a goat anti-mouse IgG coated plate so samples containing endogenous mouse IgG’s (such as mouse serum or plasma) may lead to some interference in the assay due to the likelihood for those endogenous mouse IgG’s to bind to the goat anti-mouse IgG antibody on the plate. However, this should not affect rat samples. I have provided a citation below which references using one of our PGE2 kits for rat PGE2 cell lysates.

Microsomal Prostaglandin E Synthase-1 Is a Major Terminal Synthase That Is Selectively Up-Regulated During Cyclooxygenase-2-Dependent Prostaglandin E2 Production in the Rat Adjuvant-Induced Arthritis Model: D. Claveau, et al. ; J. Immunol. 170, 4738 (2003), Application(s): EIA using rat cell lysates, http://www.jimmunol.org/cgi/content/abstract/170/9/4738;

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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