Question (73599) | Prostaglandin E2 ELISA Kit (Fluorescent) (ab136949)

Go to datasheet (ab136949)

Question

Can we offer customer

https://www.abcam.com/prostaglandin-e2nbspelisa-kit-chemiluminescent-ab136948.html

against his enquiry for Rat Prostaglandin E2, (PGE2) ELISA Kit?

Answer


ab136948 as well as our other PGE2 kits, ab133021, ab133055 and ab136949 are species independent; thus the kit is not specific for any particular species. As long as there enough PGE2 in their rat sample for the kit to detect then the kit should work assuming that the customer has optimized dilution and demonstrated dilutional linearity. ab136948 comes with a goat anti-mouse IgG coated plate so samples containing endogenous mouse IgG’s (such as mouse serum or plasma) may lead to some interference in the assay due to the likelihood for those endogenous mouse IgG’s to bind to the goat anti-mouse IgG antibody on the plate. However, this should not affect rat samples. I have provided a citation below which references using one of our PGE2 kits for rat PGE2 cell lysates.

Microsomal Prostaglandin E Synthase-1 Is a Major Terminal Synthase That Is Selectively Up-Regulated During Cyclooxygenase-2-Dependent Prostaglandin E2 Production in the Rat Adjuvant-Induced Arthritis Model: D. Claveau, et al. ; J. Immunol. 170, 4738 (2003), Application(s): EIA using rat cell lysates, http://www.jimmunol.org/cgi/content/abstract/170/9/4738;

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