Overview

  • Product name

    Prostaglandin E2 ELISA Kit (High Sensitivity )
    See all PGE2 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Low conc 24 9.8%
    Medium conc 24 6.1%
    High Conc 24 3.1%
    Inter-assay
    Sample n Mean SD CV%
    Low conc 8 12.1%
    Medium conc 8 12.6%
    High conc 8 8.1%
  • Sample type

    Cell culture supernatant, Saliva, Urine, Serum, Plasma, Whole Blood
  • Assay type

    Competitive
  • Sensitivity

    8.26 pg/ml
  • Range

    7.8 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Saliva 123.3 % - %
    Urine 108.9 % - %
    Whole Blood 101.2 % - %
    Tissue Culture Media 104.4 % - %
    Male Serum 126.1 % - %
    Female Serum 113.7 % - %

  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
    Predicted to work with: a wide range of other species
  • Product overview

    Abcam’s Prostaglandin E2 High Sensitivity in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prostaglandin E2 in serum, saliva, urine, tissue culture media and other biological fluids.


    A goat anti-mouse IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-PGE2 antigen and a monoclonal mouse antibody specific to PGE2. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of PGE2 captured in the plate.

  • Notes

    Prostaglandin E2 (PGE2) is an extensively studied prostaglandin owing to its predominance in inflammation, cancer, atherosclerosis, autoimmune disease, and sepsis. Oxidation of arachidonic acid by prostaglandin synthases (COX-1 and COX-2) produces prostaglandin H2 (PGH2), which is further metabolized by PGE synthases into its major product, PGE2. PGE2 mediates autocrine and paracrine signaling by binding to G-protein coupled receptors (EP1, EP2, EP3, EP4) on the cell surface, functioning to modulate phospholipase C and adenylate cyclase activity. PGE2 has been of great interest as a therapeutic target, either by modulation of its synthesis by COX inhibitors (NSAIDS) or by modulation of its receptors by downregulation or binding antagonists. PGE2 production in a variety of tissues has been shown to modulate numerous physiological processes including natriuresis in the kidney, smooth muscle elasticity in the vasculature, and the inflammatory response to damaged tissues by monocytes and macrophages.

     

    Compound Cross Reactivity
    PGE2 100%
    PGE1 70%
    PGE3 16.3%
    PGF1α 1.4%
    PGF2α 0.7%
    6-keto-PGF1α 0.6%
    PGA2 0.1%
    PGB1 0.1%
    13,14-dihydro-15-keto-PGF2α <0.1%
    6,15-keto-13,14-dihydro-PGF1α <0.1%
    Thromboxane B2 <0.1%
    2-Arachidonoylglycerol <0.1%
    Anandamide <0.1%
    PGD2 <0.1%
    Arachadonic Acid <0.1%
  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab133055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • PGE2 measured in human biological fluids in different ranges of dilutions (serum and EDTA-plasma at 1:5-1:50; saliva 1:1-1:5); data shown represents duplicates +/- SD. Breast milk, diluted 1:30, fell above the standard curve.  

  • PGE2 measured in cell culture medium (dilutions range 1-1:5; duplicates; +/- SD).

  • Representative Standard Curve using ab133055.

Protocols

References

This product has been referenced in:

  • Xing Y  et al. A novel piperidine identified by stem cell-based screening attenuates pulmonary arterial hypertension by regulating BMP2 and PTGS2 levels. Eur Respir J 51:N/A (2018). ELISA . Read more (PubMed: 29449428) »
  • Chen Z  et al. Epidermal growth factor and prostaglandin E2 levels in Helicobacter pylori-positive gastric intraepithelial neoplasia. J Int Med Res 44:241-7 (2016). Read more (PubMed: 26880792) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer


ab136948 as well as our other PGE2 kits, ab133021, ab133055 and ab136949 are species independent; thus the kit is not specific for any particular species. As long as there enough PGE2 in their rat sample for the kit to detect then the kit should work assuming that the customer has optimized dilution and demonstrated dilutional linearity. ab136948 comes with a goat anti-mouse IgG coated plate so samples containing endogenous mouse IgG’s (such as mouse serum or plasma) may lead to some interference in the assay due to the likelihood for those endogenous mouse IgG’s to bind to the goat anti-mouse IgG antibody on the plate. However, this should not affect rat samples. I have provided a citation below which references using one of our PGE2 kits for rat PGE2 cell lysates.

Microsomal Prostaglandin E Synthase-1 Is a Major Terminal Synthase That Is Selectively Up-Regulated During Cyclooxygenase-2-Dependent Prostaglandin E2 Production in the Rat Adjuvant-Induced Arthritis Model: D. Claveau, et al. ; J. Immunol. 170, 4738 (2003), Application(s): EIA using rat cell lysates, http://www.jimmunol.org/cgi/content/abstract/170/9/4738;

Read More

Answer

Thank you for contacting us.
The following kits can be used to determine the amount of PGE2 in tissue samples however these kits can not be used for extracting the PGE2.
https://www.abcam.com/pge2-elisa-kit-chemiluminescent-ab136948.html
https://www.abcam.com/pge2-elisa-kit-ab133021.html
https://www.abcam.com/pge2-high-sensitivity-elisa-kit-ab133055.html
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up