Anti-Prostate Specific Antigen antibody [8301] (ab403)

Abreviews (1)Q&A (18)Specific References (4)

Overview

  • Product name
    Anti-Prostate Specific Antigen antibody [8301]
    See all Prostate Specific Antigen primary antibodies
  • Description
    Mouse monoclonal [8301] to Prostate Specific Antigen
  • Host species
    Mouse
  • Specificity
    Human prostate-specific antigen - 100% PSA/alpha-1-ACT-complex - 59% Kallikrein - <0.04% PAP - <0.04% Cross-reactivity values have been calculated on weight/weight basis using 125-I-labelled human human prostate-specific antigen in assay. They can vary because of the presence of antigen or related impurities in protein preparations used for cross reactivity assays. Affinity constant: 1x1011 l/mol human prostate-specific antigen.
  • Tested applications
    Suitable for: ELISA, RIA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    native protein (purified), corresponding to amino acids 158-163 of Human Prostate Specific Antigen

Properties

Applications

Our Abpromise guarantee covers the use of ab403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
RIA
IHC-P
  • Application notes
    ELISA: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependent dilution.
    RIA: Use at an assay dependant concentration.
    Nanowires array: 1/50 - 1/500.
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function
      Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
    • Sequence similarities
      Belongs to the peptidase S1 family. Kallikrein subfamily.
      Contains 1 peptidase S1 domain.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
      • Alternative names
        • antigen, prostate-specific antibody
        • APS antibody
        • Gamma seminoprotein antibody
        • Gamma-seminoprotein antibody
        • hK3 antibody
        • Kallikrein 3 antibody
        • Kallikrein related peptidase 3 antibody
        • Kallikrein-3 antibody
        • KLK 3 antibody
        • KLK2A1 antibody
        • Klk3 antibody
        • KLK3_HUMAN antibody
        • P-30 antigen antibody
        • P30 antigen antibody
        • Prostate-specific antigen antibody
        • Psa antibody
        • Semenogelase antibody
        • Seminin antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [8301] (ab403)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [8301] (ab403)
        Human normal prostate. Staining is localised as cytoplasmic and secreted. Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control. Sections were stained using an automated system, at room temperature: sections were rehydrated and antigen retrieved with buffers (citrate pH 6.1) in a PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

      References

      This product has been referenced in:
      • Shu SK  et al. Phosphorylation and activation of androgen receptor by Aurora-A. J Biol Chem 285:33045-53 (2010). WB ; Human . Read more (PubMed: 20713353) »
      • Kim D  et al. Microarray-based multiplexed scanometric immunoassay for protein cancer markers using gold nanoparticle probes. Anal Chem 81:9183-7 (2009). Read more (PubMed: 19874062) »

      See all 4 Publications for this product

      Publishing research using ab403? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Submit a review Submit a question

      19 reviews or Q&A

      10
      Votes: Highest First

      ELISA abreview for Anti-Prostate Specific Antigen antibody [8301]

       Excellent
      Abreviews
      Application
      ELISA
      Sample
      Human Purified protein (Prostate Specific Antigen from human semen(Purity)
      Specification
      Prostate Specific Antigen from human semen(Purity
      Type
      Sandwich (Capture)
      Read More
      abreview image
      Username

      Mr. Chang Su Jeon

      Verified customer

      Submitted Jul 27 2015

      Dear technical team,


      Which form of PSA does ab403 recognize? Free PSA or complex form?

      Thank you for contacting us.

      ab403 - anti-prostate specific antigen antibody [8301] can recognize both free PSA and PSA in the form of a complex.


      I hope this information is helpful to you. Please do not hesitate to contact us if ...

      Read More

      Dear xxxx,
      Thank you very much for your help finding the reference, it is exactly what I needed.
      Do you know that if we do synthesise that particular peptide according to your website and it fails to interact with your antibody that it would st...

      Read More

      Thank you for your reply.

      I am glad you found the reference useful, I am sorry it took a little while to get to you. Unfortunately, the experiment you are planning would not be covered by the Abpromise guarantee. This is because we have not ou...

      Read More

      Dear,

      Did you manage to obtain the reference?

      Thanks,

      I am sorry for the delay in getting back to you.

      The epitope mapping performed with the Anti-Prostate Specific Antigen antibody (ab403) can be found in the reference I have attached to this email.

      The information can be found on pag...

      Read More

      We would like to try ab403 in bovine samples

      Thank you for your telephone enquiry on Friday.

      As discussed on the telephone, I am sorry that as far as we are aware, ab403 PSA antibody has never been tested with samples from cow. All tested species covered by the guarantee are stated on ...

      Read More

      Dear xxxx,

      Unfortunately there are a few confidentiality issues surrounding the project i'm working on but I'll try to give you as much information as possible.

      The target would preferably be a protein secreted in relatively large...

      Read More

      I am sorry for the delay in getting back to you.

      I have found an antibody which may work in your experiments which I am able to share the immunogen information with you. The anti-Prostate Specific Antigen antibody [PSA1] antibody ab403 was ra...

      Read More

      Dear Sir or Madam,

      I would like to order the following antibodies for the IHC-paraffin usage: anti-AMACR (ab12498), anti-androgen receptor (ab47569), anti-mitochondria (ab92824), anti-prostate specific antigen (ab403).

      We hope tho...

      Read More

      Thank you for contacting us.

      All of the antibodies you have chosen are appropriate for the experiments you are hoping to perform. All of the antibodies have been raised against the human protein but the cross reactivity with the mouse protein...

      Read More

      Dear technical team,

      Our customer purchased ab78528, ab403, ab53774 and ab9785.
      His major is "electrical engineering' and he is going toapply those itemssimilar sELISA method.
      He would like to confirm if PBS is okay as a dilut...

      Read More

      Thank you for contacting us.

      Indeed, I can confirm that PBS as dilution buffer is suitable for all three antibodies and the protein.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any mo...

      Read More

      How was the affinity constant determined?

      Thank you for your enquiry. The affinity constant was determined by RIA. In brief, according to the lab that performed this characterization: "Dilution series of antibody ab403 has been prepared and all dilutions have been incubated with 125 I ...

      Read More

      I would like to know pI (isoelectric point) value for this antibody. Do you have this information?

      Thank you for your patience in this matter. I have received a reply from the originator of ab403 (PSA antibody). The pI value of this product is 6,0 - 6,7. The assay method is PhastSystem, PhastGel IEF 3-9. I hope this information is useful for...

      Read More

      1-10 of 19 Abreviews or Q&A

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

      Sign up