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  1. Link

    prostate-specific-antigen-antibody-8301-ab403.pdf

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Neuroscience Cell Adhesion Proteins Proteases
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Anti-Prostate Specific Antigen antibody [8301] (ab403)

  • Datasheet
  • SDS
Reviews (1)Q&A (18)References (5)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [8301] (ab403)

    Key features and details

    • Mouse monoclonal [8301] to Prostate Specific Antigen
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-Prostate Specific Antigen antibody [8301]
      See all Prostate Specific Antigen primary antibodies
    • Description

      Mouse monoclonal [8301] to Prostate Specific Antigen
    • Host species

      Mouse
    • Specificity

      Human prostate-specific antigen - 100% PSA/alpha-1-ACT-complex - 59% Kallikrein - <0.04% PAP - <0.04% Cross-reactivity values have been calculated on weight/weight basis using 125-I-labelled human human prostate-specific antigen in assay. They can vary because of the presence of antigen or related impurities in protein preparations used for cross reactivity assays. Affinity constant: 1x1011 l/mol human prostate-specific antigen.
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      native protein (purified), corresponding to amino acids 158-163 of Human Prostate Specific Antigen

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 6.00
      Preservative: 0.095% Sodium azide
      Constituents: 0.335% PBS, 1.0878% Sodium citrate, 0.9% Sodium chloride
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Monoclonal
    • Clone number

      8301
    • Myeloma

      unknown
    • Isotype

      IgG1
    • Light chain type

      unknown
    • Research areas

      • Neuroscience
      • Cell Adhesion Proteins
      • Proteases
      • Tags & Cell Markers
      • Cell Type Markers
      • Tumor Associated
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • ECM Enzymes
      • Kallikreins
      • Cancer
      • Tumor immunology
      • Tumor-associated antigens
      • Cancer
      • Tumor biomarkers
      • Tumor antigens

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab403 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Notes
    Application notes
    ELISA: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependent dilution.
    RIA: Use at an assay dependant concentration.
    Nanowires array: 1/50 - 1/500.
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    Target

    • Function

      Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
    • Sequence similarities

      Belongs to the peptidase S1 family. Kallikrein subfamily.
      Contains 1 peptidase S1 domain.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P07288 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 354 Human
      • Omim: 176820 Human
      • SwissProt: P07288 Human
      • Unigene: 171995 Human
      • Alternative names

        • antigen, prostate-specific antibody
        • APS antibody
        • Gamma seminoprotein antibody
        • Gamma-seminoprotein antibody
        • hK3 antibody
        • Kallikrein 3 antibody
        • Kallikrein related peptidase 3 antibody
        • Kallikrein-3 antibody
        • KLK 3 antibody
        • KLK2A1 antibody
        • Klk3 antibody
        • KLK3_HUMAN antibody
        • P-30 antigen antibody
        • P30 antigen antibody
        • Prostate-specific antigen antibody
        • Psa antibody
        • Semenogelase antibody
        • Seminin antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [8301] (ab403)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [8301] (ab403)
        Human normal prostate. Staining is localised as cytoplasmic and secreted. Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control. Sections were stained using an automated system, at room temperature: sections were rehydrated and antigen retrieved with buffers (citrate pH 6.1) in a PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (5)

      Publishing research using ab403? Please let us know so that we can cite the reference in this datasheet.

      ab403 has been referenced in 5 publications.

      • Danthanarayana AN  et al. A multicolor multiplex lateral flow assay for high-sensitivity analyte detection using persistent luminescent nanophosphors. Anal Methods 12:272-280 (2020). PubMed: 32577135
      • Shu SK  et al. Phosphorylation and activation of androgen receptor by Aurora-A. J Biol Chem 285:33045-53 (2010). WB ; Human . PubMed: 20713353
      • Kim D  et al. Microarray-based multiplexed scanometric immunoassay for protein cancer markers using gold nanoparticle probes. Anal Chem 81:9183-7 (2009). PubMed: 19874062
      • Thaxton CS  et al. Nanoparticle-based bio-barcode assay redefines "undetectable" PSA and biochemical recurrence after radical prostatectomy. Proc Natl Acad Sci U S A 106:18437-42 (2009). Human . PubMed: 19841273
      • Zheng G  et al. Multiplexed electrical detection of cancer markers with nanowire sensor arrays. Nat Biotechnol 23:1294-301 (2005). PubMed: 16170313

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-10 of 19 Abreviews or Q&A

      ELISA abreview for Anti-Prostate Specific Antigen antibody [8301]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      ELISA
      Sample
      Human Purified protein (Prostate Specific Antigen from human semen(Purity)
      Specification
      Prostate Specific Antigen from human semen(Purity
      Type
      Sandwich (Capture)
      Read More

      Mr. Chang Su Jeon

      Verified customer

      Submitted Jul 27 2015

      Question

      Dear technical team,


      Which form of PSA does ab403 recognize? Free PSA or complex form?

      Read More

      Abcam community

      Verified customer

      Asked on Jan 17 2013

      Answer

      Thank you for contacting us.

      ab403 - anti-prostate specific antigen antibody [8301] can recognize both free PSA and PSA in the form of a complex.


      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Jan 17 2013

      Question

      Dear xxxx,
      Thank you very much for your help finding the reference, it is exactly what I needed.
      Do you know that if we do synthesise that particular peptide according to your website and it fails to interact with your antibody that it would still be covered by your abpromise return policy?
      Thanks

      Read More

      Abcam community

      Verified customer

      Asked on Oct 17 2012

      Answer

      Thank you for your reply.

      I am glad you found the reference useful, I am sorry it took a little while to get to you. Unfortunately, the experiment you are planning would not be covered by the Abpromise guarantee. This is because we have not ourselves tested the antibody in such an application (and have not recieved any information from our customers to support it) and would not therefore be able to guarantee how the antibody might perform.

      However, as we are not able to guarantee it, I can offer you a testing discount. The way this scheme works is that if you purchase the antibody as normal, test it in your binding assay and share the results with us through an Abreview (no matter whether the results are positive or negative), you would then be entitled to the value of ab403 off any products in our catalogue.

      More information on this offer can be found from the following link:

      www.abcam.com/abtrial

      The only limitation to this offer is that the antibody needs to be purchased, the Abreview submitted and the discount code used on a new order within a 4 month period.

      If you would like to participate in this scheme please do let me know as a discount code needs to be raised before the purchase of ab403.

      I hope this information has been of some help. I look forward to receiving your reply.

      Read More

      Abcam Scientific Support

      Answered on Oct 17 2012

      Question

      Dear,

      Did you manage to obtain the reference?

      Thanks,

      Read More

      Abcam community

      Verified customer

      Asked on Oct 16 2012

      Answer

      I am sorry for the delay in getting back to you.

      The epitope mapping performed with the Anti-Prostate Specific Antigen antibody (ab403) can be found in the reference I have attached to this email.

      The information can be found on page 7. The ab403 is of the clone number 8301 (ISOBM code #257) and has been mapped to the epitope group 3a. This is the region 158-163 of human Prostate Specific Antigen.

      Please note that we currently have the clone number set to PSA1 but this will be updated. The clone number should be 8301.

      I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

      Read More

      Abcam Scientific Support

      Answered on Oct 16 2012

      Question

      We would like to try ab403 in bovine samples

      Read More

      Abcam community

      Verified customer

      Asked on Oct 08 2012

      Answer

      Thank you for your telephone enquiry on Friday.

      As discussed on the telephone, I am sorry that as far as we are aware, ab403 PSA antibody has never been tested with samples from cow. All tested species covered by the guarantee are stated on the online datasheets, and these are updated as soon as any new information is brought to our attention.

      I wanted to check the alignment of the immunogen with the bovine sequence. However, I am sorry to confirm that regrettably I have been unable to find the bovine sequence in the protein database sites. I would appreciate if it is possible for you to send the bovine amino acid sequence and I will be happy to do an alignment for you so we can predict whether or not this antibody may wok on cow samples.

      If the alignment is high (> 70%), we would be pleased to provide our testing discount program. This works as follows:

      1. Reply to this e-mail to let me know that you would like to proceed and test ab403 in cow. I can then check the alignment. If the alignment is high, I can then send a discount code. This code must be issued before purchasing ab403 so please wait for my reply before ordering.

      If we are able to provide a discount code you would then proceed as follows:

      2. Purchase ab403 either by phone, fax, or online (https://www.abcam.com). This order is paid at full price.

      3. Test the antibody in cow.

      4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

      5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

      Please remember that submission of the Abreview is sufficient for the discount code to become active.

      We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab403 turns out to be unsuitable for cow, you will still receive the discount on your next purchase after your Abreview has been submitted.

      Please let me know if you have any questions about this offer and I would be happy to help you further.

      The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

      Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

      To find out more about our Abreview system, please see the following link:

      https://www.abcam.com/abreviews

      I hope this information is helpful. Please do not hesitate to contact me with the further requested details.

      Read More

      Abcam Scientific Support

      Answered on Oct 08 2012

      Question

      Dear xxxx,

      Unfortunately there are a few confidentiality issues surrounding the project i'm working on but I'll try to give you as much information as possible.

      The target would preferably be a protein secreted in relatively large quantities in seminal fluid. Ideal targets would therefore be Semenogelin or Prostate Specific Antigen. We are not sure if the material will be published in the future.

      Do you have any monoclonal antibodies for either of these targets in which a synthetic peptide immunogen sequence can be provided?

      Many thanks for all your help.

      Read More

      Abcam community

      Verified customer

      Asked on Oct 05 2012

      Answer

      I am sorry for the delay in getting back to you.

      I have found an antibody which may work in your experiments which I am able to share the immunogen information with you. The anti-Prostate Specific Antigen antibody [PSA1] antibody ab403 was raised against the native protein. However, epitope mapping has been performed to establish that the antibody binds to a region between residues 158-163 of the human protein.

      You may therefore be able to synthesise this region of the protein to perform your experiment. Do you think this antibody might be suitable for your experiment? I would like to point out that we have not tried what you are hoping to use the antibody for and would therefore not be able to predict how it might perform.

      I am waiting on a reference which details of how the epitope mapping was performed which may provide you with some useful information about different peptides used to probe the antibody with. Unfortunately, the person who has this information is not available until the middle of next week. Once I have this information I will pass it on to you.

      I hope this information has been of help. If you have any further questions, please do not hesitate to let me know.

      Read More

      Abcam Scientific Support

      Answered on Oct 05 2012

      Question

      Dear Sir or Madam,

      I would like to order the following antibodies for the IHC-paraffin usage: anti-AMACR (ab12498), anti-androgen receptor (ab47569), anti-mitochondria (ab92824), anti-prostate specific antigen (ab403).

      We hope those antibodies could exclusively identify those biomarkers in human tumor samples instead of mouse tissue. In addition, we need 300 ug for each antibody. However, I don’t know the quantity in each vial for each antibody. Please send me the quotation of sufficient vials of antibodies based on the total quantity (300 ug) I request.

      If you have better choice about above antibodies such as more quantity for each vial, cheaper for similar quality of results, or higher specificity, I would appreciate your valuable suggestions.

      Many Thanks,



      Best Regards,

      Read More

      Abcam community

      Verified customer

      Asked on Jun 07 2012

      Answer

      Thank you for contacting us.

      All of the antibodies you have chosen are appropriate for the experiments you are hoping to perform. All of the antibodies have been raised against the human protein but the cross reactivity with the mouse protein has not been ascertained and I cannot therefore say whether they will also be able to detect the mouse form of the protein.

      There are a few antibodies which may be worth considering as they may provide more experiments for the cost. All antibodies require optimisation before achieving the optimum results and it may therefore be that you will need to use more or less antibody than advised on the datasheets. The dilutions stated on the datasheets are guidelines only and will depend on a number of different factors.

      For the anti-AMACR antibody you may want to consider ab63340. This is amouse monoclonal, provided at a volume of 100 ul at the same price as ab12498 but has been shown to be usable at a dilution of1/500 to 1/2000 compared to 1/25 to 1/50 for ab12498 (500 uL provided).

      For the anti-Androgen Receptor antibody you may want to consider ab9474 (250 uL with a recommended dilution of 1/25-1/50). This should provide roughly the same number of experiments as ab47569 (100 uL with a recommended dilution of 1/50-1/100).

      For the anti-Mitochondria antibody, you may want to consider ab3298. This is provided at a volume of 500 uL with a recommended dilution of 1/500-1/1000 compared to ab92824 for which 100 uL is provided at a recommended dilution of 1/1000.

      For the anti-Prostate Specific Antigen antibody I was unable to identify another antibody which would provide you with more experiments.

      I hope this information has been of help. If you have any further questions please do not hesitate to contact us again.

      Read More

      Abcam Scientific Support

      Answered on Jun 07 2012

      Question

      Dear technical team,

      Our customer purchased ab78528, ab403, ab53774 and ab9785.
      His major is "electrical engineering' and he is going toapply those itemssimilar sELISA method.
      He would like to confirm if PBS is okay as a dilution buffer.
      Check this please.

      Read More

      Abcam community

      Verified customer

      Asked on Apr 23 2012

      Answer

      Thank you for contacting us.

      Indeed, I can confirm that PBS as dilution buffer is suitable for all three antibodies and the protein.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Apr 23 2012

      Question

      How was the affinity constant determined?

      Read More

      Abcam community

      Verified customer

      Asked on Jun 16 2008

      Answer

      Thank you for your enquiry. The affinity constant was determined by RIA. In brief, according to the lab that performed this characterization: "Dilution series of antibody ab403 has been prepared and all dilutions have been incubated with 125 I (iodine)labeled PSA antigen. Bound antibodies have been separated with PEG precipitation and counts have been measured. Amount of free antibodies has been calculated by using total max of the test run. Affinity curve has been formed from values of bound/free versus bound and then the coefficient has been calculated using slope of the curve and molecular weight of the antigen." More detailed method descriptions can be found in the literature or on the internet. I recommend a book from the Practical Approach series: Immunoassays, edited by J. P. Gosling, from Oxford University Press, 2000. I hope this information helps. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      Answered on Jun 16 2008

      Question

      I would like to know pI (isoelectric point) value for this antibody. Do you have this information?

      Read More

      Abcam community

      Verified customer

      Asked on Feb 27 2007

      Answer

      Thank you for your patience in this matter. I have received a reply from the originator of ab403 (PSA antibody). The pI value of this product is 6,0 - 6,7. The assay method is PhastSystem, PhastGel IEF 3-9. I hope this information is useful for you.

      Read More

      Abcam Scientific Support

      Answered on Mar 01 2007

      1-10 of 19 Abreviews or Q&A

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