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How was the affinity constant determined?
Asked on Jun 16 2008
Thank you for your enquiry. The affinity constant was determined by RIA. In brief, according to the lab that performed this characterization: "Dilution series of antibody ab403 has been prepared and all dilutions have been incubated with 125 I (iodine)labeled PSA antigen. Bound antibodies have been separated with PEG precipitation and counts have been measured. Amount of free antibodies has been calculated by using total max of the test run. Affinity curve has been formed from values of bound/free versus bound and then the coefficient has been calculated using slope of the curve and molecular weight of the antigen." More detailed method descriptions can be found in the literature or on the internet. I recommend a book from the Practical Approach series: Immunoassays, edited by J. P. Gosling, from Oxford University Press, 2000. I hope this information helps. Please do not hesitate to contact us if you need any more advice or information.
Answered on Jun 16 2008