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Unfortunately there are a few confidentiality issues surrounding the project i'm working on but I'll try to give you as much information as possible.
The target would preferably be a protein secreted in relatively large quantities in seminal fluid. Ideal targets would therefore be Semenogelin or Prostate Specific Antigen. We are not sure if the material will be published in the future.
Do you have any monoclonal antibodies for either of these targets in which a synthetic peptide immunogen sequence can be provided?
Many thanks for all your help.
Asked on Oct 05 2012
I am sorry for the delay in getting back to you.
I have found an antibody which may work in your experiments which I am able to share the immunogen information with you. The anti-Prostate Specific Antigen antibody [PSA1] antibody ab403 was raised against the native protein. However, epitope mapping has been performed to establish that the antibody binds to a region between residues 158-163 of the human protein.
You may therefore be able to synthesise this region of the protein to perform your experiment. Do you think this antibody might be suitable for your experiment? I would like to point out that we have not tried what you are hoping to use the antibody for and would therefore not be able to predict how it might perform.
I am waiting on a reference which details of how the epitope mapping was performed which may provide you with some useful information about different peptides used to probe the antibody with. Unfortunately, the person who has this information is not available until the middle of next week. Once I have this information I will pass it on to you.
I hope this information has been of help. If you have any further questions, please do not hesitate to let me know.
Answered on Oct 05 2012