Anti-Prostate Specific Antigen antibody [A67-B/E3] (ab2218)

Submit an Abreview Q&A (6)Specific References (1)

Overview

  • Product name
    Anti-Prostate Specific Antigen antibody [A67-B/E3]
    See all Prostate Specific Antigen primary antibodies
  • Description
    Mouse monoclonal [A67-B/E3] to Prostate Specific Antigen
  • Host species
    Mouse
  • Specificity
    This antibody is specific for PSA.
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human Prostate Specific Antigen.

Properties

Applications

Our Abpromise guarantee covers the use of ab2218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.

Target

  • Function
    Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
  • Sequence similarities
    Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • antigen, prostate-specific antibody
      • APS antibody
      • Gamma seminoprotein antibody
      • Gamma-seminoprotein antibody
      • hK3 antibody
      • Kallikrein 3 antibody
      • Kallikrein related peptidase 3 antibody
      • Kallikrein-3 antibody
      • KLK 3 antibody
      • KLK2A1 antibody
      • Klk3 antibody
      • KLK3_HUMAN antibody
      • P-30 antigen antibody
      • P30 antigen antibody
      • Prostate-specific antigen antibody
      • Psa antibody
      • Semenogelase antibody
      • Seminin antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [A67-B/E3] (ab2218)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody [A67-B/E3] (ab2218)

      Immunohistochemical analysis of human prostate tissue sections staining Prostate Specific Antigen with ab2218. 

    References

    This product has been referenced in:

    See 1 Publication for this product

    Publishing research using ab2218? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    6 reviews or Q&A

    10
    Votes: Highest First

    Bonjour Je vous remercie grandement de votre réponse. Pour répondre à vos questions concernant mon protocole de Western Blot :- J’ai bloqué la membrane avec du lait 5% dilué dans du PBS-Tween pendant 45 minutes- L’anticorps anti-PSA ab2218 a été dilué ...

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    Merci pour votre réponse.
    Pour le ab2218, je vous conseille de tester cet anticorps aussi sur des membranes bloquées avec de la BSA. Effectivement, de changer le tampon de blocage peux influencer significativement le résultat. Ceci est illust...

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    Bonjour

    Je me permets de vous recontacter suite au remplacement de mon anticorps anti-PSA par l’Ac anti-PSA ab2218.
    Cette fois-ci je vois bien une bande mais elle est totalement aspécifique puisque je retrouve le PSA chez les or...

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    Merci pour votre émail. Je suis très désolée d'entendre que le nouveau anticorps ne fonctionne pas non plus comme attendu.
    Pour l'instant nous n'avons pas eu des problèmes avec cet anticorps non plus et je ne sais pas donc ce qui peux être à l'ori...

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    Thanks for the reply. Unfortunately we cannot order a large amount. Do you mind give me the contact information of the antibody originator? I thought it would not be very difficult by NOT adding BSA. Second question: if it is impossible to get the ab...

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    Thank you for your email. I can't tell you the name of the originator of ab9537 as that information is proprietary. I double-checked ab403 and ab2218, and both of these do not contain BSA or other proteins in the storage buffer. Ab403 consists of p...

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    I saw that your company sells anti-PSA (prostate specific antigen) antibodies. Is there any possibility to receive samples so that we can test the antibodies ? Can you also provide us with information on the epitopes recognised by the different...

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    Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you d...

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    thanks for the help. Unfortunately the information on the webpage and the datasheets are not sufficient. We need information about the antibody epitopes, since we would like to detect deletions of PSA. For this detection we need an antibody that detect...

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    Regarding ab8681, the epitope is unknown. For ab2218, the originator has said that "Epitope mapping information should be found in: Summary Report on the ISOBM TD-3 Workshop, Tum. Biol. 20 (S1), pp 1-94, Eds. Rye P.D. et al. (1999). I am sure that ...

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    thanks for the help. Unfortunately the information on the webpage and the datasheets are not sufficient. We need information about the antibody epitopes, since we would like to detect deletions of PSA. For this detection we need an antibody that detect...

    Read More

    Regarding ab8681, the epitope is unknown. For ab2218, the originator has said that "Epitope mapping information should be found in: Summary Report on the ISOBM TD-3 Workshop, Tum. Biol. 20 (S1), pp 1-94, Eds. Rye P.D. et al. (1999). I am sure that ...

    Read More

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