Anti-Prostate Specific Antigen antibody [A67-B/E3] (Biotin) (ab77310)

Overview

  • Product name

    Anti-Prostate Specific Antigen antibody [A67-B/E3] (Biotin)
    See all Prostate Specific Antigen primary antibodies
  • Description

    Mouse monoclonal [A67-B/E3] to Prostate Specific Antigen (Biotin)
  • Host species

    Mouse
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    30 kDa protein fraction prepared from human seminal plasma

  • General notes

    Purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Properties

Applications

Our Abpromise guarantee covers the use of ab77310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB 1/1000. Predicted molecular weight: 29 kDa.

Target

  • Function

    Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
  • Sequence similarities

    Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • antigen, prostate-specific antibody
    • APS antibody
    • Gamma seminoprotein antibody
    • Gamma-seminoprotein antibody
    • hK3 antibody
    • Kallikrein 3 antibody
    • Kallikrein related peptidase 3 antibody
    • Kallikrein-3 antibody
    • KLK 3 antibody
    • KLK2A1 antibody
    • Klk3 antibody
    • KLK3_HUMAN antibody
    • P-30 antigen antibody
    • P30 antigen antibody
    • Prostate-specific antigen antibody
    • Psa antibody
    • Semenogelase antibody
    • Seminin antibody
    see all

References

This product has been referenced in:

  • Volpetti F  et al. A microfluidic platform for high-throughput multiplexed protein quantitation. PLoS One 10:e0117744 (2015). Read more (PubMed: 25680117) »
  • Berry SM  et al. Streamlining immunoassays with immiscible filtrations assisted by surface tension. Anal Chem 84:5518-23 (2012). Functional Studies ; Human . Read more (PubMed: 22632629) »
See all 2 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for your enquiry.

I am sorry to confirm that as far as we are aware, ab77310 has never been tested in sandwich ELISA (sELISA). It has been tested in indirect ELISA only (ELISA). All tested and guaranteed applications are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

Sandwich ELISA procedures can be difficult to optimize and tested match pair antibodies should be used. This ensures the antibodies are detecting different epitopes on the target so they do not interfere with the other antibody binding. Therefore, I regrettably we are unable to guarantee our antibodies in sandwich ELISA unless they have been specifically tested for sandwich ELISA.

Reviewing the details on the datasheet forab10184 Anti-Prostate Specific Antigen antibody [2H9], this has been tested in sELISA. Several matched pairs are suggested for use with this antibody:

'Can be paired for Sandwich ELISA with Mouse monoclonal [5A6] to Prostate Specific Antigen (ab10185) and Mouse monoclonal [8A6] to Prostate Specific Antigen (ab10187) and Mouse monoclonal [5A6] to Prostate Specific Antigen (HRP) (ab24466).

For the detection of total PSA, ab10184 can be used as a capture antibody in conjugation with ab10185 or with ab24466 (HRP-conjugated version). For the detection of free PSA, ab10184 can be used as a capture antibody in conjugation with ab10187.'

I can recommend to review the online datasheets for further information. I suggest it would be more appropriate for the customer to use one of these matched pairs suggested above with ab10184which we can guarantee for sandwich ELISA:

ab10185
https://www.abcam.com/index.html?datasheet=10185 (or use the following: https://www.abcam.com/index.html?datasheet=10185).

ab10187
https://www.abcam.com/index.html?datasheet=10187 (or use the following: https://www.abcam.com/index.html?datasheet=10187).

ab24466
https://www.abcam.com/index.html?datasheet=24466 (or use the following: https://www.abcam.com/index.html?datasheet=24466).

ab10185
https://www.abcam.com/index.html?datasheet=10185 (or use the following: https://www.abcam.com/index.html?datasheet=10185).

ab24466
https://www.abcam.com/index.html?datasheet=24466 (or use the following: https://www.abcam.com/index.html?datasheet=24466).

The ab119154 Prostate Specific Antigen proteinis of human origin and full length, and these antibodies are all tested and guarnateed for human proteins. So in theory it should work. However, please be aware this protein has not been tested and guaranteed in sELISA.

I hope this will be helpful, and if you have any further questions, please do not hesitate to contact us.

Read More

Answer

Thank you for your reply.

I have reviewed the details of this experiment and have the following feedback:

1. ab119154 Prostate Specific Antigen protein is full length human protein, so should be detected by both ab77310 and ab102885.

2. The ab102885 Easylink conjugation kit would be suitable for conjugating FITC to ab10187.

3. In theory, the experiment the customer has described should work. However, these antibodies have not been tested in this type of application, an I am sorry we would not be able to guarantee them for this.

4. Also, as discussed in the previous email, for sandwich ELISa type experiments, we would suggest to use a matched pair. ab10187 has been tested as a matched pair withthe following:

Mouse monoclonal [5A6] to Prostate Specific Antigen (ab10185)
Click here (or use the following: https://www.abcam.com/index.html?datasheet=10185).

Mouse monoclonal [8A6] to Prostate Specific Antigen (ab10187)
Click here (or use the following: https://www.abcam.com/index.html?datasheet=10187).

Mouse monoclonal [5A6] to Prostate Specific Antigen (HRP) (ab24466).
Click here (or use the following: https://www.abcam.com/index.html?datasheet=24466).
(please note this is already HRP conjugated and therefore may not be suitable)

For the detection of total PSA, ab10184 can be used as a capture antibody in conjugation with ab10185 or with ab24466 (HRP-conjugated version). For the detection of free PSA, ab10184 can be used as a capture antibody in conjugation with ab10187.)

These are unfortunately not biotin conjugated. However, we do have a biotin conjugation kits. I can recommend to review the online datasheets for further important information on how to use these kits:

ab102864 EasyLink Biotin (type A) Conjugation Kit (3 x 100µg Biotin)
https://www.abcam.com/index.html?datasheet=102864 (or use the following: https://www.abcam.com/index.html?datasheet=102864).

ab102865 EasyLink Biotin (type A) Conjugation Kit (3 x 10µg Biotin)
https://www.abcam.com/index.html?datasheet=102865 (or use the following: https://www.abcam.com/index.html?datasheet=102865).

ab102866 EasyLink Biotin (type B) Conjugation Kit (1 x 1mg Biotin)
https://www.abcam.com/index.html?datasheet=102866 (or use the following: https://www.abcam.com/index.html?datasheet=102866).

ab102867 EasyLink Biotin (type B) Conjugation Kit (3 x 100µg Biotin)
https://www.abcam.com/index.html?datasheet=102867 (or use the following: https://www.abcam.com/index.html?datasheet=102867).

I hope this information will be helpful. If you have any further questions, please do not hesitate to contact us.

Read More

Answer

Thank you for your enquiry.

Sandwich ELISA (sELISA) procedures can be difficult to optimize and tested match pair antibodies should be used. Any tested matched pairs would be noted on the datasheet. This ensures the antibodies are detecting different epitopes on the target protein so they do not interfere with the other antibody binding. Therefore, I am sorry we are unable to guarantee our antibodies in sandwich ELISA unless they have been specifically tested for sELISA.

The Prostate Specific Antigen antibody [2H9] ab10184 that your customer has found has been tested in sandwich ELISA.The information on the datasheet states that this can be paired for sELISA with one of the the following:

Mouse monoclonal [5A6] to Prostate Specific Antigen (ab10185)
https://www.abcam.com/index.html?datasheet=10185 (or use the following: https://www.abcam.com/index.html?datasheet=10185).

Mouse monoclonal [8A6] to Prostate Specific Antigen (ab10187)
https://www.abcam.com/index.html?datasheet=10187 (or use the following: https://www.abcam.com/index.html?datasheet=10187).

Mouse monoclonal [5A6] to Prostate Specific Antigen (HRP) (ab24466).
https://www.abcam.com/index.html?datasheet=24466 (or use the following: https://www.abcam.com/index.html?datasheet=24466).

For the detection of total PSA, ab10184 can be used as a capture antibody in conjugation with ab10185 or with ab24466 (HRP-conjugated version). For the detection of free PSA, ab10184 can be used as a capture antibody in conjugation with ab10187.)

I would suggest the customer selects a pair of antibodies for sELISAfrom the information above as ab77310 and ab119154 aretested in indirect ELISA only.They are not tested andguaranteedin sELISA.I would recommend using ab77310 or ab119154 in an indirect ELISA rather than sELISA.

I hope this information will be helpful. If you have any further questions, please do not hesitate to contact us.

Read More

Answer

DISCOUNT CODE:*****Expiration date: ****** I am very pleased to hear you would like to accept our offer and test ab77310 in ICC/IHC . This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ICC/IHC and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

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Question
Answer

I am very pleased to hear you would like to accept our offer and test ab77310 in ICC/IF. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

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Question
Answer

I would be very happy to issue you a discount code for ab77310. Could you please let me know on application you will test it and on what type of tissue you will be using.

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