Anti-Prostate Specific Antigen antibody (ab28563)
Key features and details
- Rabbit polyclonal to Prostate Specific Antigen
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Prostate Specific Antigen antibody
See all Prostate Specific Antigen primary antibodies -
Description
Rabbit polyclonal to Prostate Specific Antigen -
Host species
Rabbit -
Specificity
This antibody reacts with human Kallikrein 3, but does not react with other human Kallikreins (K1, 2, 4-15). -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Synthetic peptide based on the Kallikrein loop area of human Kallikrein 3. (Peptide available as ab41421.)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 33 kDa (predicted molecular weight: 29 kDa).Can be blocked with Prostate Specific Antigen peptide (ab41421).
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 33 kDa (predicted molecular weight: 29 kDa).Can be blocked with Prostate Specific Antigen peptide (ab41421). |
Target
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Function
Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. -
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- antigen, prostate-specific antibody
- APS antibody
- Gamma seminoprotein antibody
see all
Images
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All lanes : Anti-Prostate Specific Antigen antibody (ab28563) at 1/1000 dilution
Lane 1 : Kallikrein 3 at 0.05 µg
Lane 2 : Kallikrein 3 at 0.01 µg
Lane 3 : Kallikrein 3 at 0.001 µg
Predicted band size: 29 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Additional bands at: 85 kDa (possible cleavage fragment)
Datasheets and documents
References (1)
ab28563 has been referenced in 1 publication.
- McJimpsey EL Molecular Form Differences Between Prostate-Specific Antigen (PSA) Standards Create Quantitative Discordances in PSA ELISA Measurements. Sci Rep 6:22050 (2016). PubMed: 26911983