Anti-Prostate Specific Antigen antibody (ab28563)

Submit a review Q&A (1)References (1)
Western blot - Anti-Prostate Specific Antigen antibody - Kallikrein loop (ab28563)

    Key features and details

    • Rabbit polyclonal to Prostate Specific Antigen
    • Suitable for: WB
    • Reacts with: Recombinant fragment
    • Isotype: IgG

    Overview

    • Product name

      Anti-Prostate Specific Antigen antibody
      See all Prostate Specific Antigen primary antibodies

    • Description

      Rabbit polyclonal to Prostate Specific Antigen

    • Host species

      Rabbit

    • Specificity

      This antibody reacts with human Kallikrein 3, but does not react with other human Kallikreins (K1, 2, 4-15).

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Recombinant fragment

    • Immunogen

      Synthetic peptide based on the Kallikrein loop area of human Kallikrein 3. (Peptide available as ab41421.)

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab28563 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/1000 - 1/5000. Detects a band of approximately 33 kDa (predicted molecular weight: 29 kDa).Can be blocked with Prostate Specific Antigen peptide (ab41421).
    Notes
    WB
    1/1000 - 1/5000. Detects a band of approximately 33 kDa (predicted molecular weight: 29 kDa).Can be blocked with Prostate Specific Antigen peptide (ab41421).

    Target

    • Function

      Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

    • Sequence similarities

      Belongs to the peptidase S1 family. Kallikrein subfamily.
      Contains 1 peptidase S1 domain.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Alternative names

      • antigen, prostate-specific antibody
      • APS antibody
      • Gamma seminoprotein antibody
      • Gamma-seminoprotein antibody
      • hK3 antibody
      • Kallikrein 3 antibody
      • Kallikrein related peptidase 3 antibody
      • Kallikrein-3 antibody
      • KLK 3 antibody
      • KLK2A1 antibody
      • Klk3 antibody
      • KLK3_HUMAN antibody
      • P-30 antigen antibody
      • P30 antigen antibody
      • Prostate-specific antigen antibody
      • Psa antibody
      • Semenogelase antibody
      • Seminin antibody
      see all

    Images

    • Western blot - Anti-Prostate Specific Antigen antibody - Kallikrein loop (ab28563)
      Western blot - Anti-Prostate Specific Antigen antibody - Kallikrein loop (ab28563)
      All lanes : Anti-Prostate Specific Antigen antibody (ab28563) at 1/1000 dilution

      Lane 1 : Kallikrein 3 at 0.05 µg
      Lane 2 : Kallikrein 3 at 0.01 µg
      Lane 3 : Kallikrein 3 at 0.001 µg

      Predicted band size: 29 kDa
      Observed band size: 34 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 85 kDa (possible cleavage fragment)

    References (1)

    Publishing research using ab28563? Please let us know so that we can cite the reference in this datasheet.

    ab28563 has been referenced in 1 publication.

    • McJimpsey EL Molecular Form Differences Between Prostate-Specific Antigen (PSA) Standards Create Quantitative Discordances in PSA ELISA Measurements. Sci Rep 6:22050 (2016). PubMed: 26911983

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question


    Human PSA protein and anti-PSA antibody

    I have found many products related to PSA(prostrate specific antigen), one product has been found , having its antibody with it. the catalog # no is (ab 41421) , product description : prostrate specific antigen peptide (Kallikrein loop), used as an immunogen for (ab 28563-kallikrein 3Ab-kallikrein loop.), .

    2nd product is PSA peptide (244-254) (ab 45800) having seq. c-termina lhuman PSA, it is used as an immunogen for ab 39983-PSA Ab. but this antibody product is not available.Can you please suggest me any alternate for this .

    Please also confirm about their specific reactivity with each other.

    Read More
    Answer


    Another antibody/peptide combination is Anti-Prostate Specific Antigen antibody - Catalytic domain (ab40949) / Prostate Specific Antigen peptide (Catalytic domain) (ab43085).
    Regarding your question: "Please also confirm about their specific reactivity with each other."
    I think you are wondering if any of the peptides you mention will react with the other PSA antibodies. For the specific antibody/peptide pairs you note, the antibodies will be specific only for their cognate peptide. These peptide immunogens are short, 10-20 amino acid peptides.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com