Product nameAnti-Prostate Specific Antigen antibody
See all Prostate Specific Antigen primary antibodies
DescriptionRabbit polyclonal to Prostate Specific Antigen
SpecificityThis antibody detects endogenous levels of total Prostate Specific Antigen protein.
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide derived from human Prostate Specific Antigen
- Extracts from mouse heart
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab53774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 29 kDa).|
|IHC-P||Use at an assay dependent concentration.|
FunctionHydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain.
- Information by UniProt
- antigen, prostate-specific antibody
- APS antibody
- Gamma seminoprotein antibody
All lanes : Anti-Prostate Specific Antigen antibody (ab53774) at 1/500 dilution
Lane 1 : Extracts from mouse heart
Lane 2 : Extracts from mouse heart, plus immunising peptide
Predicted band size: 29 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Ab53774 staining human normal prostate tissue. Staining is localised to cytoplasm and extracellular spaces.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be requir
This product has been referenced in:
- Coleman DJ et al. Maintenance of MYC expression promotes de novo resistance to BET bromodomain inhibition in castration-resistant prostate cancer. Sci Rep 9:3823 (2019). Read more (PubMed: 30846826) »
- Hua Y et al. Dual androgen receptor (AR) and STAT3 inhibition by a compound targeting the AR amino-terminal domain. Pharmacol Res Perspect 6:e00437 (2018). Read more (PubMed: 30410767) »