Anti-Prostate Specific Antigen antibody (ab53774)
- Datasheet
- Specific References (4)
- Protocols
Overview
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Product nameAnti-Prostate Specific Antigen antibody
See all Prostate Specific Antigen primary antibodies -
DescriptionRabbit polyclonal to Prostate Specific Antigen
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Host speciesRabbit
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SpecificityThis antibody detects endogenous levels of total Prostate Specific Antigen protein.
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Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Synthetic peptide derived from human Prostate Specific Antigen
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Positive control
- Extracts from mouse heart
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab53774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 29 kDa). | |
| ELISA | 1/20000. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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FunctionHydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
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Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 354 Human
- Entrez Gene: 16621 Mouse
- Omim: 176820 Human
- SwissProt: P07288 Human
- SwissProt: P00756 Mouse
- Unigene: 171995 Human
- Unigene: 439740 Mouse
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Alternative names
- antigen, prostate-specific antibody
- APS antibody
- Gamma seminoprotein antibody
see all
Images
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Western blot - Anti-Prostate Specific Antigen antibody (ab53774)All lanes : Anti-Prostate Specific Antigen antibody (ab53774) at 1/500 dilution
Lane 1 : Extracts from mouse heart
Lane 2 : Extracts from mouse heart, plus immunising peptide
Predicted band size: 29 kDa
Observed band size: 34 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody (ab53774)Ab53774 staining human normal prostate tissue. Staining is localised to cytoplasm and extracellular spaces.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be requir
References
This product has been referenced in:
- Olsen JR et al. Context dependent regulatory patterns of the androgen receptor and androgen receptor target genes. BMC Cancer 16:377 (2016). WB ; Human . Read more (PubMed: 27378372) »
- Volpetti F et al. A microfluidic platform for high-throughput multiplexed protein quantitation. PLoS One 10:e0117744 (2015). Read more (PubMed: 25680117) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"