Anti-Prostate Specific Antigen antibody (ab53774)

Reviews (1)Q&A (4)References (6)

Overview

  • Product name
    Anti-Prostate Specific Antigen antibody
    See all Prostate Specific Antigen primary antibodies
  • Description
    Rabbit polyclonal to Prostate Specific Antigen
  • Host species
    Rabbit
  • Specificity
    This antibody detects endogenous levels of total Prostate Specific Antigen protein.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from human Prostate Specific Antigen

  • Positive control
    • Extracts from mouse heart

Properties

Applications

Our Abpromise guarantee covers the use of ab53774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 29 kDa).
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
  • Sequence similarities
    Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • antigen, prostate-specific antibody
      • APS antibody
      • Gamma seminoprotein antibody
      • Gamma-seminoprotein antibody
      • hK3 antibody
      • Kallikrein 3 antibody
      • Kallikrein related peptidase 3 antibody
      • Kallikrein-3 antibody
      • KLK 3 antibody
      • KLK2A1 antibody
      • Klk3 antibody
      • KLK3_HUMAN antibody
      • P-30 antigen antibody
      • P30 antigen antibody
      • Prostate-specific antigen antibody
      • Psa antibody
      • Semenogelase antibody
      • Seminin antibody
      see all

    Images

    • Western blot - Anti-Prostate Specific Antigen antibody (ab53774)
      Western blot - Anti-Prostate Specific Antigen antibody (ab53774)
      All lanes : Anti-Prostate Specific Antigen antibody (ab53774) at 1/500 dilution

      Lane 1 : Extracts from mouse heart
      Lane 2 : Extracts from mouse heart, plus immunising peptide

      Predicted band size: 29 kDa
      Observed band size: 34 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody (ab53774)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostate Specific Antigen antibody (ab53774)
      Ab53774 staining human normal prostate tissue. Staining is localised to cytoplasm and extracellular spaces.
      Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be requir

    References

    This product has been referenced in:
    See all 6 Publications for this product

    Publishing research using ab53774? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-Prostate Specific Antigen antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (LNCaP prostate cancer cells)
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Loading amount
    25 µg
    Specification
    LNCaP prostate cancer cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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    Abcam user community

    Verified customer

    Submitted Nov 19 2018

    Question

    Dear technical team,

    Our customer purchased ab78528, ab403, ab53774 and ab9785.
    His major is "electrical engineering' and he is going toapply those itemssimilar sELISA method.
    He would like to confirm if PBS is okay as a dilution buffer.
    Check this please.

    Read More
    Answer

    Thank you for contacting us.

    Indeed, I can confirm that PBS as dilution buffer is suitable for all three antibodies and the protein.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    I have a question on your anti-Prostate Specific Antigen antibody with Cat. No ab53774. I wonder what the sequence of the immunogen is. Do you also make customized antibody for a customer? If yes, what is your price? Thank you.

    Read More
    Answer

    Thank you for contacting Abcam.

    Theimmunogen sequence for ab53774is from ****** of human Prostate Specific Antigen.

    We continually add antibodies and other protein detection products to our catalogue but we do not offer custom-made antibodies. However, we are happy to pass on the contact details of the following companies that do offer this service:

    United States

    Pocono Rabbit Farm and Laboratory (PRF&L)
    PRF&L
    PO Box 240
    Canadensis, PA 18325
    Tel: 800-622-6381 - Toll Free in the United States & Canada
    Tel: 570-595-7108 - Worldwide
    Fax: 570-595-9277
    Website: www.prfal.com

    Maine Biotechnology Services, Inc.
    1037R Forest Ave.
    Portland Maine 04103
    Tel: 800-925-9476 or 207-797-5454
    Fax: 207-797-5595
    Website: www.mainebiotechnology.com

    Cocalico Biologicals, Inc.
    PO Box 265
    449 Stevens Road
    Reamstown, PA 17567-0265
    Tel: 717-336-1990
    Fax: 717-336-1993
    Website: www.cocalicobiologicals.com

    United Kingdom

    Charles River Laboratories International, Inc.
    Tel: +44 (0) 1843 823575
    Website: www.criver.com

    I hope this information is helpful to you. Please do not hesitate to contact us if you have any questions.

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    Question

    I did see this note on the data sheet, but this antibody AB39983 does not exist  any more.  As I understand the customer needs a PSA antigen and an anti PSA antibvody reacting with that antigen.  Have you got anything?

    Read More
    Answer

    You are correct that ab39983 has been discontinued. I am sorry about that. I should have checked all products were still available. Here are 2 more product recommendations for your customer: Anti-Prostate Specific Antigen antibody (ab53774) will react with Prostate Specific Antigen protein (Human) (ab78528). I hope this helps. Please let me know if you need any further assistance.

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    Question

    I will like to know urgently if your biotech company can synthesize a prostate specific antigen which is for anti-prostate specific antigen antibody so that we can place the order immediately. I gave him a quote for the 2 following products: AB45800, PSA peptide AB53774, rabbit pAb to PSA. He wants to know if the antibody would bind to the antigen. Thank you in advance for your help.

    Read More
    Answer

    Thank you for your enquiry. Based on the researcher's notes, I'm not quite sure what the customer is interested in doing with their experiment. But regarding your question, I don't think ab53774 will react with the PSA peptide ab45800. As noted on the datasheet for ab45800, it is the immunogen used to produce a the anti-PSA antibody, catalog number ab39983. So ab39983 will bind to ab45800. I hope this is helpful. Please contact me again if you have any further questions.

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