Overview

  • Product name
    Anti-Prostate Specific Antigen antibody
    See all Prostate Specific Antigen primary antibodies
  • Description
    Rabbit polyclonal to Prostate Specific Antigen
  • Host species
    Rabbit
  • Specificity
    This antibody detects endogenous levels of total Prostate Specific Antigen protein.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from human Prostate Specific Antigen

  • Positive control
    • Extracts from mouse heart

Properties

Applications

Our Abpromise guarantee covers the use of ab53774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 29 kDa).
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
  • Sequence similarities
    Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen, prostate-specific antibody
    • APS antibody
    • Gamma seminoprotein antibody
    • Gamma-seminoprotein antibody
    • hK3 antibody
    • Kallikrein 3 antibody
    • Kallikrein related peptidase 3 antibody
    • Kallikrein-3 antibody
    • KLK 3 antibody
    • KLK2A1 antibody
    • Klk3 antibody
    • KLK3_HUMAN antibody
    • P-30 antigen antibody
    • P30 antigen antibody
    • Prostate-specific antigen antibody
    • Psa antibody
    • Semenogelase antibody
    • Seminin antibody
    see all

Images

  • All lanes : Anti-Prostate Specific Antigen antibody (ab53774) at 1/500 dilution

    Lane 1 : Extracts from mouse heart
    Lane 2 : Extracts from mouse heart, plus immunising peptide

    Predicted band size: 29 kDa
    Observed band size: 34 kDa
    why is the actual band size different from the predicted?

  • Ab53774 staining human normal prostate tissue. Staining is localised to cytoplasm and extracellular spaces.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be requir

References

This product has been referenced in:
See all 6 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (LNCaP prostate cancer cells)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
25 µg
Specification
LNCaP prostate cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Nov 19 2018

Answer

Thank you for contacting us.

Indeed, I can confirm that PBS as dilution buffer is suitable for all three antibodies and the protein.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting Abcam.

Theimmunogen sequence for ab53774is from ****** of human Prostate Specific Antigen.

We continually add antibodies and other protein detection products to our catalogue but we do not offer custom-made antibodies. However, we are happy to pass on the contact details of the following companies that do offer this service:

United States

Pocono Rabbit Farm and Laboratory (PRF&L)
PRF&L
PO Box 240
Canadensis, PA 18325
Tel: 800-622-6381 - Toll Free in the United States & Canada
Tel: 570-595-7108 - Worldwide
Fax: 570-595-9277
Website: www.prfal.com

Maine Biotechnology Services, Inc.
1037R Forest Ave.
Portland Maine 04103
Tel: 800-925-9476 or 207-797-5454
Fax: 207-797-5595
Website: www.mainebiotechnology.com

Cocalico Biologicals, Inc.
PO Box 265
449 Stevens Road
Reamstown, PA 17567-0265
Tel: 717-336-1990
Fax: 717-336-1993
Website: www.cocalicobiologicals.com

United Kingdom

Charles River Laboratories International, Inc.
Tel: +44 (0) 1843 823575
Website: www.criver.com

I hope this information is helpful to you. Please do not hesitate to contact us if you have any questions.

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Answer

You are correct that ab39983 has been discontinued. I am sorry about that. I should have checked all products were still available. Here are 2 more product recommendations for your customer: Anti-Prostate Specific Antigen antibody (ab53774) will react with Prostate Specific Antigen protein (Human) (ab78528). I hope this helps. Please let me know if you need any further assistance.

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Answer

Thank you for your enquiry. Based on the researcher's notes, I'm not quite sure what the customer is interested in doing with their experiment. But regarding your question, I don't think ab53774 will react with the PSA peptide ab45800. As noted on the datasheet for ab45800, it is the immunogen used to produce a the anti-PSA antibody, catalog number ab39983. So ab39983 will bind to ab45800. I hope this is helpful. Please contact me again if you have any further questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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