Product nameAnti-Prostate Specific Antigen antibody [EP1588Y]
See all Prostate Specific Antigen primary antibodies
DescriptionRabbit monoclonal [EP1588Y] to Prostate Specific Antigen
SpecificityThe antibody provided good results in western blot when tested in human prostate lysate. Unfortunately, when 22RV1 cell lysate was used no band was detected and inconsistent results were obtained with LNCaP cell lysate (where different lots of cell lysate were used for the test). Please contact our Scientific Support if you have any feedback and/or questions.
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Synthetic peptide within Human Prostate Specific Antigen aa 200-300 (C terminal). The exact sequence is proprietary.
Database link: P07288
- WB: Human prostate carcinoma tissue lysate. IHC-P: Human prostate carcinoma tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Dissociation constant (KD)KD = 5.04 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab76113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 34 kDa (predicted molecular weight: 29 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionHydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain.
- Information by UniProt
- antigen, prostate-specific antibody
- APS antibody
- Gamma seminoprotein antibody
All lanes : Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113) at 1/1000 dilution
Lane 1 : Human prostate tissue lysate
Lane 2 : LNCaP whole cell lysate
Lane 3 : 22Rv1 whole cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Exposure time -
Lane 1: 15 seconds.
Lanes 2-3: 3 minutes.
Blocking and dilution buffer: 5% NFDM/TBST.
ab76113, at 1/100 dilution, staining Prostate Specific Antigen in paraffin-embedded human prostate carcinoma tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Li F et al. XPNPEP2 is associated with lymph node metastasis in prostate cancer patients. Sci Rep 9:10078 (2019). Read more (PubMed: 31296901) »
- Yao J et al. Bioconjugated, Single-Use Biosensor for the Detection of Biomarkers of Prostate Cancer. ACS Omega 3:6411-6418 (2018). Read more (PubMed: 30023946) »