Overview

  • Product name

    Anti-Prostate Specific Antigen antibody [SP317]
    See all Prostate Specific Antigen primary antibodies
  • Description

    Rabbit monoclonal [SP317] to Prostate Specific Antigen
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Prostate Specific Antigen (internal sequence). The exact sequence is proprietary.
    Database link: P07288

  • Positive control

    • WB: LNCaP cell lysate. IHC-P: Human prostate tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab224799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Predicted molecular weight: 29 kDa.
IHC-P 1/100.

Perform heat mediated antigen retrieval with EDTA pH 8.0 buffer before commencing with IHC staining protocol.

Target

  • Function

    Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
  • Sequence similarities

    Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • antigen, prostate-specific antibody
    • APS antibody
    • Gamma seminoprotein antibody
    • Gamma-seminoprotein antibody
    • hK3 antibody
    • Kallikrein 3 antibody
    • Kallikrein related peptidase 3 antibody
    • Kallikrein-3 antibody
    • KLK 3 antibody
    • KLK2A1 antibody
    • Klk3 antibody
    • KLK3_HUMAN antibody
    • P-30 antigen antibody
    • P30 antigen antibody
    • Prostate-specific antigen antibody
    • Psa antibody
    • Semenogelase antibody
    • Seminin antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate tissue sections labeling Prostate Specific Antigen with ab224799 at 1/100 dilution (3.83 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on the human prostate, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab224799 for 10 mins at room temperature.

  • Anti-Prostate Specific Antigen antibody [SP317] (ab224799) at 1/100 dilution + LNCaP (human prostate cancer cell line) cell lysate

    Predicted band size: 29 kDa

  • Formalin-fixed, paraffin-embedded human prostate tissue stained for Prostate Specific Antigen using ab224799 at 1/100 dilution in immunohistochemical analysis.

References

ab224799 has not yet been referenced specifically in any publications.

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