Key features and details
- Rabbit monoclonal [SP317] to Prostate Specific Antigen
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Prostate Specific Antigen antibody [SP317]
See all Prostate Specific Antigen primary antibodies
DescriptionRabbit monoclonal [SP317] to Prostate Specific Antigen
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Prostate Specific Antigen (internal sequence). The exact sequence is proprietary.
Database link: P07288
- WB: LNCaP cell lysate. IHC-P: Human prostate tissue.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact firstname.lastname@example.org.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from TCS by protein A/G.
Our Abpromise guarantee covers the use of ab224799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100. Predicted molecular weight: 29 kDa.|
Perform heat mediated antigen retrieval with EDTA pH 8.0 buffer before commencing with IHC staining protocol.
FunctionHydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain.
- Information by UniProt
- antigen, prostate-specific antibody
- APS antibody
- Gamma seminoprotein antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate tissue sections labeling Prostate Specific Antigen with ab224799 at 1/100 dilution (3.83 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on the human prostate, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab224799 for 10 mins at room temperature.
Anti-Prostate Specific Antigen antibody [SP317] (ab224799) at 1/100 dilution + LNCaP (human prostate cancer cell line) cell lysate
Predicted band size: 29 kDa
Formalin-fixed, paraffin-embedded human prostate tissue stained for Prostate Specific Antigen using ab224799 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224799 has not yet been referenced specifically in any publications.