Overview

  • Product name

    Anti-Prosurfactant Protein C antibody
    See all Prosurfactant Protein C primary antibodies
  • Description

    Rabbit polyclonal to Prosurfactant Protein C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human SFTPC.
    Database link: P11686

  • Positive control

    • U2OS cells; Jurkat, U937 and Raji cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab196677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 21 kDa.
ICC/IF 1/50 - 1/200.
IHC-P 1/50 - 1/100.

Target

  • Function

    Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
  • Involvement in disease

    Defects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2) [MIM:610913]; also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. A rare disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course, due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.
    Genetic variations in SFTPC are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS) [MIM:267450]; also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'.
  • Sequence similarities

    Contains 1 BRICHOS domain.
  • Cellular localization

    Secreted > extracellular space > surface film.
  • Information by UniProt
  • Database links

  • Alternative names

    • BRICD6 antibody
    • BRICHOS domain containing 6 antibody
    • PSP C antibody
    • PSPC antibody
    • PSPC_HUMAN antibody
    • Pulmonary surfactant apoprotein 2 antibody
    • Pulmonary surfactant apoprotein PSP C antibody
    • pulmonary surfactant apoprotein-2 SP-C antibody
    • Pulmonary surfactant associated protein C antibody
    • Pulmonary surfactant associated proteolipid SPL pVal antibody
    • Pulmonary surfactant associated proteolipid SPL(Val) antibody
    • Pulmonary surfactant protein SP5 antibody
    • Pulmonary surfactant-associated protein C antibody
    • Pulmonary surfactant-associated proteolipid SPL(Val) antibody
    • SFTP 2 antibody
    • SFTP2 antibody
    • SFTPC antibody
    • SFTPC surfactant pulmonary associated protein C antibody
    • SMDP2 antibody
    • SP 5 antibody
    • SP C antibody
    • SP-C antibody
    • SP5 antibody
    • SPC antibody
    • Surfactant associated protein pulmonary 2 antibody
    • Surfactant protein c antibody
    • Surfactant proteolipid SPL-pVal antibody
    • Surfactant pulmonary associated protein C antibody
    see all

Images

  • All lanes : Anti-Prosurfactant Protein C antibody (ab196677) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : U937 cell extract
    Lane 3 : Raji cell extract

    Predicted band size: 21 kDa

  • Immunofluorescent analysis of U2OS cells labeling SFTPC with ab196677 at 1/50 dilution. Lower image with DAPI for nuclear staining.

References

This product has been referenced in:

  • Guenthart BA  et al. Regeneration of severely damaged lungs using an interventional cross-circulation platform. Nat Commun 10:1985 (2019). Read more (PubMed: 31064987) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse whole lung cells, isolated with intent to us)
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris NuPAGE)
Loading amount
50 µg
Specification
Mouse whole lung cells, isolated with intent to us
Blocking step
Odyssey TBS Blocking Buffer (proprietary) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C

Pete Serbedzija

Verified customer

Submitted Apr 11 2016

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