• Product name

    Anti-Prosurfactant Protein C antibody
    See all Prosurfactant Protein C primary antibodies
  • Description

    Rabbit polyclonal to Prosurfactant Protein C
  • Host species

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human, Pig
  • Immunogen

    Recombinant fragment corresponding to Human Prosurfactant Protein C aa 1-35.

  • Positive control

    • WB: Human lung tissue lysate. IHC-P: Mouse lung tissue.



Our Abpromise guarantee covers the use of ab40879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.


IHC-P Use at an assay dependent concentration.
WB 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 23350749


  • Function

    Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
  • Involvement in disease

    Defects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2) [MIM:610913]; also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. A rare disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course, due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.
    Genetic variations in SFTPC are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS) [MIM:267450]; also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'.
  • Sequence similarities

    Contains 1 BRICHOS domain.
  • Cellular localization

    Secreted > extracellular space > surface film.
  • Information by UniProt
  • Database links

  • Alternative names

    • BRICD6 antibody
    • BRICHOS domain containing 6 antibody
    • PSP C antibody
    • PSPC antibody
    • PSPC_HUMAN antibody
    • Pulmonary surfactant apoprotein 2 antibody
    • Pulmonary surfactant apoprotein PSP C antibody
    • pulmonary surfactant apoprotein-2 SP-C antibody
    • Pulmonary surfactant associated protein C antibody
    • Pulmonary surfactant associated proteolipid SPL pVal antibody
    • Pulmonary surfactant associated proteolipid SPL(Val) antibody
    • Pulmonary surfactant protein SP5 antibody
    • Pulmonary surfactant-associated protein C antibody
    • Pulmonary surfactant-associated proteolipid SPL(Val) antibody
    • SFTP 2 antibody
    • SFTP2 antibody
    • SFTPC antibody
    • SFTPC surfactant pulmonary associated protein C antibody
    • SMDP2 antibody
    • SP 5 antibody
    • SP C antibody
    • SP-C antibody
    • SP5 antibody
    • SPC antibody
    • Surfactant associated protein pulmonary 2 antibody
    • Surfactant protein c antibody
    • Surfactant proteolipid SPL-pVal antibody
    • Surfactant pulmonary associated protein C antibody
    see all


  • Anti-Prosurfactant Protein C antibody (ab40879) at 1/500 dilution + Lung (Human) Tissue Lysate at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 21 kDa
    Observed band size: 21 kDa
    Additional bands at: 38 kDa, 49 kDa, 79 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 90 seconds

    Secondary antibody - goat anti rabbit HRP (pre-adsorbed-ab97080)

  • ab40879 staining Prosurfactant Protein C in A549 cells from Human lung tissue by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were incubated with primary antibody (1/250). A FITC-conjugated anti-rabbit (1/200) was used as the secondary antibody. DAPI was used to detect the nucleus of the cells.

  • Immunohistochemical analysis of Pig lung tissue, labeling prosurfactant protein C with ab40879. Tissue fixed in 10% buffered formalin for 4 hours, and paraffin embedded. Anitgen retrieval performed in buffer at pH 9 at 75°C for 20 minutes. Blocked with PBS, treated with primary antibody, followed by secondary, and DAPI stained. 

  • Adult mouse lung was embedded in paraffin and stained with anti-N terminal proSP-C rabbit serum ab40879 (1/2000 dilution).


This product has been referenced in:

  • Zhang C  et al. CXCR4-Overexpressing Umbilical Cord Mesenchymal Stem Cells Enhance Protection against Radiation-Induced Lung Injury. Stem Cells Int 2019:2457082 (2019). Read more (PubMed: 30867667) »
  • Lehmann M  et al. Differential effects of Nintedanib and Pirfenidone on lung alveolar epithelial cell function in ex vivo murine and human lung tissue cultures of pulmonary fibrosis. Respir Res 19:175 (2018). Read more (PubMed: 30219058) »
See all 22 Publications for this product

Customer reviews and Q&As

Flow Cytometry
Human Cell (lung)
Cell harvesting/tissue preparation method: trypsinization
Sample buffer: 3% FBS in PBS
Yes - 0.1% saponin
Gating Strategy
dead cells were gated

Ms. maayan burshtein

Verified customer

Submitted Sep 09 2011

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