Anti-Prosurfactant Protein C antibody (ab90716)

Rabbit polyclonal Prosurfactant Protein C antibody. Validated in WB, IP, IHC, ICC/IF and tested in Mouse, Human. Cited in 19 publication(s). Independently reviewed in 5 review(s).

Overview

  • Product name

    Anti-Prosurfactant Protein C antibody
    See all Prosurfactant Protein C primary antibodies
  • Description

    Rabbit polyclonal to Prosurfactant Protein C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Human Prosurfactant Protein C aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab99881)

  • Positive control

    • Recombinant Human Prosurfactant Protein C (ab114293) can be used as a positive control in WB. This antibody gave a positive signal in the Human and Mouse Lung Tissue lysates. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: A549. This antibody gave a positive result in IHC in the following FFPE tissue: human normal lung.

Properties

Applications

Our Abpromise guarantee covers the use of ab90716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 21 kDa).
IP Use a concentration of 5 µg/ml.

Target

  • Function

    Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
  • Involvement in disease

    Defects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2) [MIM:610913]; also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. A rare disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course, due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.
    Genetic variations in SFTPC are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS) [MIM:267450]; also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'.
  • Sequence similarities

    Contains 1 BRICHOS domain.
  • Cellular localization

    Secreted > extracellular space > surface film.
  • Information by UniProt
  • Database links

  • Alternative names

    • BRICD6 antibody
    • BRICHOS domain containing 6 antibody
    • PSP C antibody
    • PSPC antibody
    • PSPC_HUMAN antibody
    • Pulmonary surfactant apoprotein 2 antibody
    • Pulmonary surfactant apoprotein PSP C antibody
    • pulmonary surfactant apoprotein-2 SP-C antibody
    • Pulmonary surfactant associated protein C antibody
    • Pulmonary surfactant associated proteolipid SPL pVal antibody
    • Pulmonary surfactant associated proteolipid SPL(Val) antibody
    • Pulmonary surfactant protein SP5 antibody
    • Pulmonary surfactant-associated protein C antibody
    • Pulmonary surfactant-associated proteolipid SPL(Val) antibody
    • SFTP 2 antibody
    • SFTP2 antibody
    • SFTPC antibody
    • SFTPC surfactant pulmonary associated protein C antibody
    • SMDP2 antibody
    • SP 5 antibody
    • SP C antibody
    • SP-C antibody
    • SP5 antibody
    • SPC antibody
    • Surfactant associated protein pulmonary 2 antibody
    • Surfactant protein c antibody
    • Surfactant proteolipid SPL-pVal antibody
    • Surfactant pulmonary associated protein C antibody
    see all

Images

  • IHC image of Prosurfactant Protein C staining in a section of formalin-fixed paraffin-embedded normal human lung performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab90716, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

     

  • All lanes : Anti-Prosurfactant Protein C antibody (ab90716) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Lung (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 21 kDa
    Observed band size: 25,28 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes
  • All lanes : Anti-Prosurfactant Protein C antibody (ab90716) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate


    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 21 kDa
    Observed band size: 25,28 kDa why is the actual band size different from the predicted?
    Additional bands at: 45 kDa (possible non-specific binding)


    Exposure time: 2 minutes
  • ab90716 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab90716 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Prosurfactant Protein C was immunoprecipitated using 0.5mg Mouse Lung tissue, 5µg of Rabbit polyclonal to Prosurfactant Protein C and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Lung tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab90716.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 23kDa; Prosurfactant Protein C

References

This product has been referenced in:

  • Umezawa K  et al. Phospholipase Ce plays a crucial role in neutrophilic inflammation accompanying acute lung injury through augmentation of CXC chemokine production from alveolar epithelial cells. Respir Res 20:9 (2019). Read more (PubMed: 30634975) »
  • Nguyen TM  et al. The proportion of alveolar type 1 cells decreases in murine hypoplastic congenital diaphragmatic hernia lungs. PLoS One 14:e0214793 (2019). Read more (PubMed: 30995255) »
See all 22 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Antigen retrieval step
None
Permeabilization
No
Specification
Lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 07 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (lung, mixed)
Permeabilization
No
Specification
lung, mixed
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 23°C
Fixative
Paraformaldehyde

Miss. Kara Shumansky

Verified customer

Submitted May 16 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Lungs)
Permeabilization
No
Specification
Lungs
Fixative
Formaldehyde

Mr. Alexis Broquet

Verified customer

Submitted May 23 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (lung)
Permeabilization
No
Specification
lung
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 14 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Lung)
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris NuPAGE)
Loading amount
40 µg
Specification
Lung
Blocking step
Odyssey TBS Blocking Buffer (proprietary) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C

Pete Serbedzija

Verified customer

Submitted May 25 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (lung)
Permeabilization
Yes - 0.1% Triton X-100
Specification
lung
Blocking step
10% Serum + 0.1% Triton X in PBS at room temperature as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 16 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
None
Sample
Mouse Tissue sections (Lung)
Specification
Lung
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 25 2014

Answer

Thank you for contacting us.

These product are Immunogen affinity purified rabbit IgG polyclonal antibodies, not serum.

I also wanted to clarify a point about the 100% testing discount codes. Only one code may be used per vial purchased. So if you were to test all four conditions, you would need to purchase four vials of antibodies. Its rather like a buy-one, test, get-one free system.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

I am very happy to hear of your interest in testing these product. If you should decide to proceed I may generate a 100% tesdt code for each tested application of each product. So if you purchase both products and test each in IHC and in Flow Cytometry you will recieve four Abreview 100% testing discount codes entitling you to 4 primary antibodies of your choice after you submit your Abreviews.

Unfortunately the isotype control is not included in this offer as these are known to perform in the applications.

I've included more information below. Please let me know if you have any other questions.

Thank you very much for your interest in ab90716 and ab101251.

To our knowledge,these have not been tested in IHC or Flow Cytometry. Therefore, I can offer a discount off a future purchase if you buy now, test it inIHC or Flowand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY per code.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and testthese inWB and/or Flow. I will then send a discount code. This code must be issued before purchasing so please wait for my reply before ordering.

2. Purchase either by phone, fax, or online (https://www.abcam.com). Please reference the codes when purchasing.

3. Test it in WB and/or Flow.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifa productturns out to be unsuitable for the application, you will still receive the discount on your next purchase after your Abreview has been submitted.


The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for contacting us.

We have not tested these products in applications other than Western blot and cannot guarantee that these will work in IHC or in Flow Cytometry. If you are interested in trying these products in those assays on tissues that we have determined that the antibody will cross react with, we could offer you our 100% Abreview testing discount. In this program, I would product aabreview code for you for each of the products you wish to test, you would then purchase the product at regular price.
You would thentest the product in your species and application and submit your results as an Abreview (https://www.abcam.com/abreviews). Once the Abreview is submitted, the discount code will become active and would entitle you to a free antibody of your choice.

If you are interested in this or if you have any questions please to not hesitate to contact me.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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