Anti-Prosurfactant Protein C antibody (ab90716)
Rabbit polyclonal Prosurfactant Protein C antibody. Validated in WB, IP, IHC, ICC/IF and tested in Mouse, Human. Cited in 19 publication(s). Independently reviewed in 5 review(s).
- Datasheet
- References (22)
- Protocols
Overview
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Product name
Anti-Prosurfactant Protein C antibody
See all Prosurfactant Protein C primary antibodies -
Description
Rabbit polyclonal to Prosurfactant Protein C -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rhesus monkey
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Immunogen
Synthetic peptide corresponding to Human Prosurfactant Protein C aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab99881) -
Positive control
- Recombinant Human Prosurfactant Protein C (ab114293) can be used as a positive control in WB. This antibody gave a positive signal in the Human and Mouse Lung Tissue lysates. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: A549. This antibody gave a positive result in IHC in the following FFPE tissue: human normal lung.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab90716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| IHC-P | Use a concentration of 1 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 21 kDa). | |
| IP | Use a concentration of 5 µg/ml. |
Target
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Function
Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. -
Involvement in disease
Defects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2) [MIM:610913]; also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. A rare disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course, due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.
Genetic variations in SFTPC are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS) [MIM:267450]; also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'. -
Sequence similarities
Contains 1 BRICHOS domain. -
Cellular localization
Secreted > extracellular space > surface film. - Information by UniProt
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Database links
- Entrez Gene: 6440 Human
- Entrez Gene: 20389 Mouse
- Omim: 178620 Human
- SwissProt: P11686 Human
- SwissProt: P21841 Mouse
- Unigene: 1074 Human
- Unigene: 24040 Mouse
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Alternative names
- BRICD6 antibody
- BRICHOS domain containing 6 antibody
- PSP C antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody (ab90716)IHC image of Prosurfactant Protein C staining in a section of formalin-fixed paraffin-embedded normal human lung performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab90716, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-Prosurfactant Protein C antibody (ab90716)All lanes : Anti-Prosurfactant Protein C antibody (ab90716) at 1 µg/ml
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Lung (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 25,28 kDa why is the actual band size different from the predicted?
Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Western blot - Anti-Prosurfactant Protein C antibody (ab90716)All lanes : Anti-Prosurfactant Protein C antibody (ab90716) at 1 µg/ml
Lane 1 : Lung (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 25,28 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa (possible non-specific binding)
Exposure time: 2 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Prosurfactant Protein C antibody (ab90716)ab90716 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab90716 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunoprecipitation - Anti-Prosurfactant Protein C antibody (ab90716)Prosurfactant Protein C was immunoprecipitated using 0.5mg Mouse Lung tissue, 5µg of Rabbit polyclonal to Prosurfactant Protein C and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Lung tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab90716.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 23kDa; Prosurfactant Protein C
Protocols
Datasheets and documents
References
This product has been referenced in:
- Umezawa K et al. Phospholipase Ce plays a crucial role in neutrophilic inflammation accompanying acute lung injury through augmentation of CXC chemokine production from alveolar epithelial cells. Respir Res 20:9 (2019). Read more (PubMed: 30634975) »
- Nguyen TM et al. The proportion of alveolar type 1 cells decreases in murine hypoplastic congenital diaphragmatic hernia lungs. PLoS One 14:e0214793 (2019). Read more (PubMed: 30995255) »
