Anti-Prosurfactant Protein C antibody (ab90716)
- Datasheet
- Specific References (15)
- Protocols
Overview
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Product nameAnti-Prosurfactant Protein C antibody
See all Prosurfactant Protein C primary antibodies -
DescriptionRabbit polyclonal to Prosurfactant Protein C
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IHC-P, WB, IPmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rhesus monkey
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Prosurfactant Protein C.
(Peptide available as ab99881.)
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Positive control
- This antibody gave a positive signal in the Human and Mouse Lung Tissue lysates. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: A549. This antibody gave a positive result in IHC in the following FFPE tissue: human normal lung.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab90716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| IHC-P | Use a concentration of 1 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 21 kDa). | |
| IP | Use a concentration of 5 µg/ml. |
Target
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FunctionPulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
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Involvement in diseaseDefects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2) [MIM:610913]; also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. A rare disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course, due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.
Genetic variations in SFTPC are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS) [MIM:267450]; also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'. -
Sequence similaritiesContains 1 BRICHOS domain.
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Cellular localizationSecreted > extracellular space > surface film.
- Information by UniProt
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Database links
- Entrez Gene: 6440 Human
- Entrez Gene: 20389 Mouse
- Omim: 178620 Human
- SwissProt: P11686 Human
- SwissProt: P21841 Mouse
- Unigene: 1074 Human
- Unigene: 24040 Mouse
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Alternative names
- BRICD6 antibody
- BRICHOS domain containing 6 antibody
- PSP C antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody (ab90716)IHC image of Prosurfactant Protein C staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90716, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-Prosurfactant Protein C antibody (ab90716)All lanes : Anti-Prosurfactant Protein C antibody (ab90716) at 1 µg/ml
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Lung (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 25,28 kDa (why is the actual band size different from the predicted?)
Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Prosurfactant Protein C antibody (ab90716)ab90716 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab90716 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunoprecipitation - Anti-Prosurfactant Protein C antibody (ab90716)Prosurfactant Protein C was immunoprecipitated using 0.5mg Mouse Lung tissue, 5µg of Rabbit polyclonal to Prosurfactant Protein C and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Lung tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab90716.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 23kDa; Prosurfactant Protein C
Protocols
References
This product has been referenced in:
- Fiaturi N et al. CCN5 in alveolar epithelial proliferation and differentiation during neonatal lung oxygen injury. J Cell Commun Signal 12:217-229 (2018). Read more (PubMed: 29349730) »
- Mikamo M et al. Inhibiting Skp2 E3 Ligase Suppresses Bleomycin-Induced Pulmonary Fibrosis. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29415439) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"