Overview

  • Product name
    Anti-Prosurfactant Protein C antibody - Aminoterminal end
    See all Prosurfactant Protein C primary antibodies
  • Description
    Rabbit polyclonal to Prosurfactant Protein C - Aminoterminal end
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Prosurfactant Protein C aa 1-30 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P11686

  • Positive control
    • WB: Mouse liver tissue lysate ICC/IF: HepG2 cells.
  • General notes

    This antibody can detect the membrane bound form, not the mature form. It detects SFTPC in the cytoplasm before propeptide (1-23aa) is removed from golgi.

Properties

Applications

Our Abpromise guarantee covers the use of ab170699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 21 kDa.
ICC/IF 1/25.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
  • Involvement in disease
    Defects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2) [MIM:610913]; also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. A rare disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course, due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.
    Genetic variations in SFTPC are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS) [MIM:267450]; also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'.
  • Sequence similarities
    Contains 1 BRICHOS domain.
  • Cellular localization
    Secreted > extracellular space > surface film.
  • Information by UniProt
  • Database links
  • Alternative names
    • BRICD6 antibody
    • BRICHOS domain containing 6 antibody
    • PSP C antibody
    • PSPC antibody
    • PSPC_HUMAN antibody
    • Pulmonary surfactant apoprotein 2 antibody
    • Pulmonary surfactant apoprotein PSP C antibody
    • pulmonary surfactant apoprotein-2 SP-C antibody
    • Pulmonary surfactant associated protein C antibody
    • Pulmonary surfactant associated proteolipid SPL pVal antibody
    • Pulmonary surfactant associated proteolipid SPL(Val) antibody
    • Pulmonary surfactant protein SP5 antibody
    • Pulmonary surfactant-associated protein C antibody
    • Pulmonary surfactant-associated proteolipid SPL(Val) antibody
    • SFTP 2 antibody
    • SFTP2 antibody
    • SFTPC antibody
    • SFTPC surfactant pulmonary associated protein C antibody
    • SMDP2 antibody
    • SP 5 antibody
    • SP C antibody
    • SP-C antibody
    • SP5 antibody
    • SPC antibody
    • Surfactant associated protein pulmonary 2 antibody
    • Surfactant protein c antibody
    • Surfactant proteolipid SPL-pVal antibody
    • Surfactant pulmonary associated protein C antibody
    see all

Images

  • Anti-Prosurfactant Protein C antibody - Aminoterminal end (ab170699) + Mouse liver tissue lysate at 35 µg

    Predicted band size: 21 kDa

  • Immunocytochemsitry/Immunofluorescence analysis of HepG2 cells labeling Prosurfactant Protein C (green) using ab170699. An Alexa Fluor® 488-conjugated goat anti-rabbit lgG was used as the secondary antibody. Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).

References

This product has been referenced in:
  • Liu X  et al. Cell type specific expression of Follistatin-like 1 (Fstl1) in mouse embryonic lung development. J Mol Histol N/A:N/A (2018). Read more (PubMed: 29916090) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
CyTOF
Sample
Mouse Cell (Lung)
Specification
Lung

Irit Milman

Verified customer

Submitted Nov 08 2017

Application
Flow Cytometry
Permeabilization
No
Sample
Human Cell (Primary human alveolar epithelial type II cells (A)
Specification
Primary human alveolar epithelial type II cells (A
Gating Strategy
gated on anti-SPC-APC
Preparation
Cell harvesting/tissue preparation method: ATII cells were isolated from normal human lung tissue following collagenase and trypsin digestion, DNase I treatment, Percoll density gradient centrifugation, filtration through 40um cell strainers, and depletion of alveolar macrophages using anti-CD14 antibody. 1 - 2 x 10^6 Cells were then washed with FACS buffer (PBS containing 1% BSA and 0.02% NaN3) three times in 96-well U bottom plate by centrifugation. Cells were then stained with Anti-Prosurfactant Protein C antibody (ab170699) (1:15) for 30min at 4oC in the dark, followed by secondary antibody, sheep anti-rabbit IgG Dylight649 (1:100 AbD Serotec) in FACS buffer for 30min at 4oC in the dark. Cells were washed with FACS buffer three times before FACS analysis.
Sample buffer: FACS buffer (PBS containing 1% BSA and 0.02% NaN3)

Siying Ye

Verified customer

Submitted Mar 24 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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