Recombinant
RabMAb

Recombinant Anti-Protamine 2 antibody [EPR15738] (ab190791)

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Overview

  • Product name

    Anti-Protamine 2 antibody [EPR15738]
    See all Protamine 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR15738] to Protamine 2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Protamine 2 aa 1-100. The exact sequence is proprietary.
    Database link: P04554

  • Positive control

    • Human testis lysate.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15738

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 13 kDa).

Target

  • Relevance

    Protamines are small, arginine-rich proteins involved in the condensation of sperm chromatin. During spermiogenesis, histones are replaced by protamines (P1 and P2), resulting in sperm chromatin condensation followed by a halt to gene expression in haploid spermatids and spermatozoa. As a consequence, protamine deficiency and aberrant P1/P2 ratio have a profound effect on both fertilization and embryo development.

  • Cellular localization

    Nuclear

  • Database links

    • Alternative names

      • Basic nuclear protein HPI1 antibody
      • Basic nuclear protein HPI2 antibody
      • Basic nuclear protein HPS1 antibody
      • Basic nuclear protein HPS2 antibody
      • Cancer/testis antigen family 94 member 2 antibody
      • CT94.2 antibody
      • PRM2 antibody
      • Protamine P2 HP2 antibody
      • Sperm histone HP2 antibody
      • Sperm histone HP3 antibody
      • Sperm histone HP4 antibody
      • Sperm histone P2 antibody
      • Sperm protamine P2 antibody
      • Sperm protamine P3 antibody
      • Sperm protamine P4 antibody
      see all

    Images

    • Western blot - Anti-Protamine 2 antibody [EPR15738] (ab190791)
      Western blot - Anti-Protamine 2 antibody [EPR15738] (ab190791)
      Anti-Protamine 2 antibody [EPR15738] (ab190791) at 1/10000 dilution + Human testis lysate at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 13 kDa
      Observed band size: 17 kDa
      why is the actual band size different from the predicted?

    References

    ab190791 has not yet been referenced specifically in any publications.

    Publishing research using ab190791? Please let us know so that we can cite the reference in this datasheet.

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