Protease Activity Assay Kit (Fluorometric - Red) (ab112153)


  • Product name
    Protease Activity Assay Kit (Fluorometric - Red)
    See all Protease kits
  • Sample type
    Plasma, Cell culture extracts, Other biological fluids, Whole Blood
  • Assay type
  • Assay time
    1h 00m
  • Product overview

    Protease assays are widely used for the investigation of protease inhibitors and detection of protease activities. Monitoring various protease activities has become a routine task for many biological laboratories. Some proteases have been identified as good drug development targets.

    ab112153 Protease Activity Assay Kit is an ideal choice to perform routine assays for the isolation of proteases, or for identifying the presence of contaminating proteases in protein samples.
    ab112153 uses a red fluorescent casein conjugate that is proven to be a generic substrate for a broad spectrum of proteases (e.g. trypsin, chymotrypsin, thermolysin, proteinase K, protease XIV, and elastase). In the intact substrate, casein is heavily labeled with a fluorescent dye, resulting in significant fluorescence quenching. Protease-catalyzed hydrolysis relieves its quenching effect, yielding brightly fluorescent dye-labeled short peptides. The increase in fluorescence intensity is directly proportional to protease activity. The assay can be performed in a convenient 96-well or 384-well microtiter plate format. Its signal can be easily read at Ex/Em = 540 /590 nm.
    ab112153 has been used for screening protease inhibitors in a HTS mode.

    Visit our FAQs page for tips and troubleshooting.



  • Trypsin protease activity was analyzed by using ab112153. Protease substrate was incubated with 3 units of trypsin in the kit assay buffer. The control wells had protease substrate only (without trypsin). The fluorescence signal was measured starting from time 0 when trypsin was added using a fluorescence microplate reader with a filter set of Ex/Em = 540/590 nm. Samples were done in triplicate.



ab112153 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you very much for your reply.

I am checking with the lab regarding the upper limit of the kits, and I will get back to you as soon as I have that information.

I might recommend the kit ab111750 out of these three, as it has be...

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Thank you again for your patience while I have been in touch with the lab regarding these kits.

The kit ab111750 should not be used with turbid or non-clear samples, but my contact at the lab did say that ab112152 or ab112153 might be ok to u...

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Thank you very much for contacting us with your question.

We have only tested these kits with biological samples at this time, and we do not have information about their use with environmental samples. Could you tell me a little bit more abou...

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For ab112153, the Protease Activity Assay kit. The products has a shelf life of 6 months from the date or purchase. I am still waiting to hear back on ab102523 and will let you know as soon as I hear back.


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