For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Thank you for your swift reply. The samples are centrifuged and the supernatant is 0.2µm-filtered. However, in some cases, centrifugation does not produce any supernatant and the substrate is too viscous for filtration. Even after dilution and above treatment there might remain humic acid in the sample and turbidity that can influence results. Experiences with enzyme activities in slurries,manure, compost are very limited and results not standardized. I guess activities are in the range of ng-µg peptides/g dm per hr or ng-µg peptides/ml per hr.
Indeed I have another question: while the expected activities seem to match with your kits, it would be useful to know how "wide" the linear part of your standard curve is.
I suppose that your lower limit is somewhere at 2ng/mlxhr. What is your upper limit in the same sample?
The reason is that we assume that enzyme activities, in manure for example, differ considerably depending on the samples. Preparation of time consuming dilution series could be prevented.
I suppose we are going to test some kits available on the market. What are the chances that we get "sample" assay kits to run some tests instead of buying 100-500 test kits with your company?
Asked on Aug 28 2012
Thank you very much for your reply.
I am checking with the lab regarding the upper limit of the kits, and I will get back to you as soon as I have that information.
I might recommend the kit ab111750 out of these three, as it has been tested with the widest range of samples (including food samples).However I'm concerned that the turbid or viscous samples would not be suitable for use withany of thekits, as clear samples are recommended and the measurements may not be accurate otherwise.I have also asked the lab if they have any data or opinions in this regard.
We only have the full size kits available for sale, so unfortunately there are no samples to send out. However, if you would like to try one these kits, I can offer a 15% discount to try one. Would you be interested in this?
I look forward to hearing from you, and I will be back in touch as soon as I have more information from the lab.
Answered on Aug 28 2012