Protease and Phosphatase Inhibitor Cocktail (ab201119)

Overview

  • Product name

    Protease and Phosphatase Inhibitor Cocktail
  • Product overview

    To protect against both protease and phosphatase in one solution try our dual cocktail (EDTA-free also available).

    AEBSF free and mass spectroscopy compatible – water soluble.

    Target proteases - serine proteases, aminopeptidase B, leucine aminopeptidase, cysteine proteases, metalloproteases

    Target phosphatases - serine/threonine phosphatases, acid phosphatases, protein tyrosine phosphatases, alkaline phosphatases

    Inhibitors included - aprotinin (bovine lung), bestatin, E-64, leupeptin hemisulfate, β-glycerophosphate, sodium fluoride, sodium orthovanadate, sodium pyrophosphate decahydrate, EDTA disodium salt.

    Form: Supplied as 1 vial of cocktail in water (1 ml) and 1 vial of EDTA disodium salt (0.5 mM in 1 ml of water)

    Preparation: The recommended starting dilution is 1:9

     

    Visit our inhibitor cocktails page for more information on our complete range of convenient and easy to use protease and phosphatase inhibitor cocktails.

  • Notes

    Individual components and concentration:

    Aprotinin (bovine lung): serine protease inhibitor (0.08 mM)
    Bestatin: aminopeptidase B and leucine aminopeptidase inhibitor (5 mM)
    E-64: cysteine protease inhibitor (1.5 mM)
    Leupeptin hemisulfate: serine/cysteine protease inhibitor (2 mM)
    β-Glycerophosphate: serine/threonine phosphatase inhibitor (10 mM)
    Sodium fluoride: acid phosphatase and serine/threonine phosphatase inhibitor (50 mM)
    Sodium orthovanadate: protein tyrosine phosphatase and alkaline phosphatase inhibitor (1 mM)
    Sodium pyrophosphate decahydrate: serine/threonine phosphatase inhibitor (10 mM)
    EDTA disodium salt: metalloprotease inhibitor (0.5 mM)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 kit
    EDTA Solution (10X) Clear 1 x 1ml
    Protease and Phosphatase Inhibitor Cocktail (10X) Red 1 x 1ml

References

This product has been referenced in:

  • Lodge EJ  et al. Homeostatic and tumourigenic activity of SOX2+ pituitary stem cells is controlled by the LATS/YAP/TAZ cascade. Elife 8:N/A (2019). Read more (PubMed: 30912742) »
  • You Q  et al. MicroRNA-650 targets inhibitor of growth 4 to promote colorectal cancer progression via mitogen activated protein kinase signaling. Oncol Lett 16:2326-2334 (2018). Read more (PubMed: 30008936) »
See all 6 Publications for this product

Customer reviews and Q&As

Answer

All the inhibitors in the ab201119 Protease and Phosphatase Inhibitor Cocktail kit including EDTA are present in 10X concentration. The final concentration of the inhibitors in the lysate should be 1x.  EDTA solution is added to the samples in such a way that the final concentration is 1X.

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