Protease and Phosphatase Inhibitor Cocktail (ab201119)
Overview
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Product name
Protease and Phosphatase Inhibitor Cocktail -
Product overview
To protect against both protease and phosphatase in one solution try our dual cocktail (EDTA-free also available).
AEBSF free and mass spectroscopy compatible – water soluble.
Target proteases - serine proteases, aminopeptidase B, leucine aminopeptidase, cysteine proteases, metalloproteases
Target phosphatases - serine/threonine phosphatases, acid phosphatases, protein tyrosine phosphatases, alkaline phosphatases
Inhibitors included - aprotinin (bovine lung), bestatin, E-64, leupeptin hemisulfate, β-glycerophosphate, sodium fluoride, sodium orthovanadate, sodium pyrophosphate decahydrate, EDTA disodium salt.
Form: Supplied as 1 vial of cocktail in water (1 ml) and 1 vial of EDTA disodium salt (0.5 mM in 1 ml of water)
Preparation: The recommended starting dilution is 1:9
Visit our inhibitor cocktails page for more information on our complete range of convenient and easy to use protease and phosphatase inhibitor cocktails.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K283 EZBlock™ Universal Protease and Phosphatase Inhibitor Cocktail. K283-1 is the same size as the 1 kit size of ab201119.
Individual components and concentration:
Aprotinin (bovine lung): serine protease inhibitor (0.08 mM)
Bestatin: aminopeptidase B and leucine aminopeptidase inhibitor (5 mM)
E-64: cysteine protease inhibitor (1.5 mM)
Leupeptin hemisulfate: serine/cysteine protease inhibitor (2 mM)
β-Glycerophosphate: serine/threonine phosphatase inhibitor (10 mM)
Sodium fluoride: acid phosphatase and serine/threonine phosphatase inhibitor (200 mM)
Sodium orthovanadate: protein tyrosine phosphatase and alkaline phosphatase inhibitor (1 mM)
Sodium pyrophosphate decahydrate: serine/threonine phosphatase inhibitor (10 mM)
EDTA disodium salt: metalloprotease inhibitor (0.5 mM)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 kit EDTA Solution (10X) 1 x 1ml Protease and Phosphatase Inhibitor Cocktail (10X) 1 x 1ml
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (36)
ab201119 has been referenced in 36 publications.
- Qiu N et al. IFT20 Confers Paclitaxel Resistance by Triggering β-arrestin-1 to Modulate ASK1 Signaling in Breast Cancer. Mol Cancer Res 21:214-227 (2023). PubMed: 36573960
- Liu L et al. TRPA1 protects mice from pathogenic Citrobacter rodentium infection via maintaining the colonic epithelial barrier function. FASEB J 37:e22739 (2023). PubMed: 36583647
- Yao W et al. ERVWE1 Reduces Hippocampal Neuron Density and Impairs Dendritic Spine Morphology through Inhibiting Wnt/JNK Non-Canonical Pathway via miR-141-3p in Schizophrenia. Viruses 15:N/A (2023). PubMed: 36680208
- Leahy SN et al. FMRP activity and control of Csw/SHP2 translation regulate MAPK-dependent synaptic transmission. PLoS Biol 21:e3001969 (2023). PubMed: 36701299
- Li X et al. HERV-W ENV Induces Innate Immune Activation and Neuronal Apoptosis via linc01930/cGAS Axis in Recent-Onset Schizophrenia. Int J Mol Sci 24:N/A (2023). PubMed: 36769337