Product nameProtease and Phosphatase Inhibitor Cocktail (EDTA-free)
To protect against both protease and phosphatase in one solution try our dual cocktail (also available with EDTA).
AEBSF free and mass spectroscopy compatible – water soluble.
Target proteases - serine proteases, aminopeptidase B, leucine aminopeptidase, cysteine proteases
Target phosphatases - Serine/threonine phosphatases, acid phosphatases, protein tyrosine phosphatases, alkaline phosphatases
Inhibitors included - aprotinin (bovine lung), bestatin, E-64, leupeptin hemisulfate, β-glycerophosphate, sodium fluoride, sodium orthovanadate, sodium pyrophosphate decahydrate
Form: Supplied in water (1 ml)
Preparation: The recommended starting dilution is 1:9
Visit our inhibitor cocktails page for more information on our complete range of convenient and easy to use protease and phosphatase inhibitor cocktails.
Individual components and concentrations:
Aprotinin (bovine lung): serine protease inhibitor (0.08 mM)
Bestatin: aminopeptidase B and leucine aminopeptidase inhibitor (5 mM)
E-64: cysteine protease inhibitor (1.5 mM)
Leupeptin hemisulfate: serine/cysteine protease inhibitor (2 mM)
β-Glycerophosphate: serine/threonine phosphatase inhibitor (10 mM)
Sodium fluoride: acid phosphatase and serine/threonine phosphatase inhibitor (50 mM)
Sodium orthovanadate: protein tyrosine phosphatase and alkaline phosphatase inhibitor (1 mM)
Sodium pyrophosphate decahydrate: serine/threonine phosphatase inhibitor (10 mM)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 ml Protease and Phosphatase Inhibitor Cocktail (EDTA-free) 1 x 1ml