Protease and Phosphatase Inhibitor Cocktail (EDTA-free) (ab201120)

Overview

  • Product name

    Protease and Phosphatase Inhibitor Cocktail (EDTA-free)
  • Product overview

    To protect against both protease and phosphatase in one solution try our dual cocktail (also available with EDTA).

    AEBSF free and mass spectroscopy compatible – water soluble.

    Target proteases - serine proteases, aminopeptidase B, leucine aminopeptidase, cysteine proteases

    Target phosphatases - Serine/threonine phosphatases, acid phosphatases, protein tyrosine phosphatases, alkaline phosphatases

    Inhibitors included - aprotinin (bovine lung), bestatin, E-64, leupeptin hemisulfate, β-glycerophosphate, sodium fluoride, sodium orthovanadate, sodium pyrophosphate decahydrate

    Form: Supplied in water (1 ml)

    Preparation: The recommended starting dilution is 1:9

     

    Visit our inhibitor cocktails page for more information on our complete range of convenient and easy to use protease and phosphatase inhibitor cocktails.

  • Notes

    Individual components and concentrations:

    Aprotinin (bovine lung): serine protease inhibitor (0.08 mM)
    Bestatin: aminopeptidase B and leucine aminopeptidase inhibitor (5 mM)
    E-64: cysteine protease inhibitor (1.5 mM)
    Leupeptin hemisulfate: serine/cysteine protease inhibitor (2 mM)
    β-Glycerophosphate: serine/threonine phosphatase inhibitor (10 mM)
    Sodium fluoride: acid phosphatase and serine/threonine phosphatase inhibitor (50 mM)
    Sodium orthovanadate: protein tyrosine phosphatase and alkaline phosphatase inhibitor (1 mM)
    Sodium pyrophosphate decahydrate: serine/threonine phosphatase inhibitor (10 mM) 

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 ml
    Protease and Phosphatase Inhibitor Cocktail (EDTA-free) 1 x 1ml

References

This product has been referenced in:

  • Hu TY  et al. Anti-inflammation action of xanthones from Swertia chirayita by regulating COX-2/NF-?B/MAPKs/Akt signaling pathways in RAW 264.7 macrophage cells. Phytomedicine 55:214-221 (2019). Read more (PubMed: 30668431) »
See 1 Publication for this product

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