Product nameProtease Inhibitor Cocktail
Abcam's Protease Inhibitor Cocktail contains a mixture of water-soluble protease inhibitors with broad specificity for various proteases, and has been optimized to generate the maximal protection to mammalian tissue and cell extracts.
Supplied as a lyophilized powder.
One vial can make 125 ml of cocktail solution.
Add 250 μl DMSO to the vial, pipette several times to dissolve all powder (This makes 500X concentrated inhibitor cocktail).
Visit our FAQs page for tips and troubleshooting.
Crude cell extracts contain many endogeneous enzymes, such as proteases and phosphotases, which can degrade valuable proteins in the extract. The individual components of the protease inhibitor cocktail have specific inhibitory properties. A description of each inhibitor is given below:
Aprotinin: Inhibits serine proteases, such as trypsin, chymotrypsin, plasmin, trypsinogen, urokinase, and kallikrein.
Leupeptin: Inhibits both serine and cysteine proteases, such as calpain, trypsin, papain, and cathepsin B.
Pepstain A: Inhibits acid proteases, such as pepsin, rennin, cathepsin D, chymosin, and protease B.
PMSF: Inhibits serine proteases, such as trypsin and chymotrypsin. Also inhibits cysteine proteases and mammalian acetylcholinesterase.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 kit Protease Inhibitor Cocktail 1 vial
RelevanceCrude cell extracts contain many endogeneous enzymes, such as proteases and phosphotases, which can degrade valuable proteins in an extract. A protease inhibitor cocktail contains a mixture of protease inhibitors with broad specificity for various proteases.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab65621 has been referenced in 15 publications.
- Wang D et al. Galectin-3 regulates chemotherapy sensitivity in epithelial ovarian carcinoma via regulating mitochondrial function. J Toxicol Sci 44:47-56 (2019). PubMed: 30626779
- Huang H et al. M1 Macrophage Activated by Notch Signal Pathway Contributed to Ventilator-Induced Lung Injury in Chronic Obstructive Pulmonary Disease Model. J Surg Res 244:358-367 (2019). PubMed: 31323391
- Li G et al. Tumor suppressor function of miR-129-5p in lung cancer. Oncol Lett 17:5777-5783 (2019). PubMed: 31186804
- El Khoury P et al. Proteomic analysis of a Candida albicans pir32 null strain reveals proteins involved in adhesion, filamentation and virulence. PLoS One 13:e0194403 (2018). PubMed: 29554112
- Zhang J et al. Oncogenic role of microRNA-532-5p in human colorectal cancer via targeting of the 5'UTR of RUNX3. Oncol Lett 15:7215-7220 (2018). PubMed: 29849790