Overview

  • Product name

    Protease Inhibitor Cocktail
  • Product overview

    Abcam's Protease Inhibitor Cocktail contains a mixture of water-soluble protease inhibitors with broad specificity for various proteases, and has been optimized to generate the maximal protection to mammalian tissue and cell extracts.


    Form:


    Supplied as a lyophilized powder.


    One vial can make 125 ml of cocktail solution.


    Preparation:


    Add 250 μl DMSO to the vial, pipette several times to dissolve all powder (This makes 500X concentrated inhibitor cocktail).


    Click here for EDTA-free version of this cocktail.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Crude cell extracts contain many endogeneous enzymes, such as proteases and phosphotases, which can degrade valuable proteins in the extract. The individual components of the protease inhibitor cocktail have specific inhibitory properties. A description of each inhibitor is given below:

    Aprotinin: Inhibits serine proteases, such as trypsin, chymotrypsin, plasmin, trypsinogen, urokinase, and kallikrein.

    Leupeptin: Inhibits both serine and cysteine proteases, such as calpain, trypsin, papain, and cathepsin B.

    Pepstain A: Inhibits acid proteases, such as pepsin, rennin, cathepsin D, chymosin, and protease B.

    PMSF: Inhibits serine proteases, such as trypsin and chymotrypsin. Also inhibits cysteine proteases and mammalian acetylcholinesterase.

     

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 kit
    Protease Inhibitor Cocktail 1 vial
  • Research areas

  • Relevance

    Crude cell extracts contain many endogeneous enzymes, such as proteases and phosphotases, which can degrade valuable proteins in an extract. A protease inhibitor cocktail contains a mixture of protease inhibitors with broad specificity for various proteases.
  • Alternative names

    • Aprotinin
    • Leupeptin
    • Pepstatin
    • PMSF

References

This product has been referenced in:

  • Wang D  et al. Galectin-3 regulates chemotherapy sensitivity in epithelial ovarian carcinoma via regulating mitochondrial function. J Toxicol Sci 44:47-56 (2019). Read more (PubMed: 30626779) »
  • Huang H  et al. M1 Macrophage Activated by Notch Signal Pathway Contributed to Ventilator-Induced Lung Injury in Chronic Obstructive Pulmonary Disease Model. J Surg Res 244:358-367 (2019). Read more (PubMed: 31323391) »
See all 14 Publications for this product

Customer reviews and Q&As

1-10 of 15 Abreviews or Q&A

Answer

Thank you for contacting us.  To reconstitute this product, add 250-µl DMSO to the vial and pipet several times to dissolve all powder (This makes 500X concentrated inhibitor cocktail).  I have added these instructions to our datasheet.  I hope this helps, please let me know if you need any additional information. 

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Question
Answer

I can confirm that ab65621 Protease Inhibitor Cocktail can be used on saliva samples.

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Answer


I can confirm that ab65621 Protease Inhibitor Cocktail contains EDTA.

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Answer

The protease inhibitor cocktail can be stored in small aliquots at -20C after reconstitution in DMSO for 1 year. We recommend to avoid freezing and thawing during this time.

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Answer


After reconstituting in DMSO, the cocktail should be stored at -20C.

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Answer


The laboratory said you can reconstitute ab65621 in 250 µl of DMSO. This makes it 250 µl of 500X inhibitor cocktail. Which means that for every 500 µl of your sample can add 1 µl of the inhibitor. These reconstitution instructions are applicable for serum, plasma, and lysate samples.

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Answer

Thank you for contacting us.


The alternatives are as follows

1. ab103746; https://www.abcam.com/BrightMount-25ml-Aqueous-Mounting-Medium-for-Fluorescent-Staining-ab103746.html

or ab128982; https://www.abcam.com/Aqueous-Mounting-Medium-ab128982.html

2. We do not sell Poly L Lysine




5. ab133412; https://www.abcam.com/Nitrocellulose-Transfer-Membrane-045-um-ab133412.html
6. We have similar product;https://www.abcam.com/Nitrocellulose-Transfer-Membrane-045-um-ab133412.html
7. Similar product;https://www.abcam.com/Nitrocellulose-Transfer-Membrane-045-um-ab133412.html
8. We sell similar but as cocktail; https://www.abcam.com/Protease-Inhibitor-Cocktail-ab65621.html

9. We sell similar but as cocktail; https://www.abcam.com/Protease-Inhibitor-Cocktail-ab65621.html

10. We sell similar but as cocktail; https://www.abcam.com/Protease-Inhibitor-Cocktail-ab65621.html

11. We do not sell SAP

12. We don have clone 4A6 antibody. We however have ab32; https://www.abcam.com/c-Myc-antibody-9E10-ChIP-Grade-ab32.htmlwhich is better than clone 4A6.

13. We do not sell protein purification kit Ni-NTA column Cat.No.31014 (50 columns)

14. We do not sell protein purification kit protein purification kit Ni-NTA slurry Cat. No.30250

15. We do not sell NEB chitin-intein Tag cat # E6901S

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us. To the best of our knowledge the SDS is compliant with Canadian legislation as long as it has the statement This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations (CPR) and the MSDS contains all the information required by the CPR. in section 15. I have attached the updated SDS with this statement for you. I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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Answer

Thank you for contacting us.

Could you please provide a catalog number for this product? I did find that we do carry a Protease Inhibitors and have attached the SDS from our datasheet for that product (ab65621) however if this is not the correct product please let me know and I will locate the appropriate SDS.

This link will direct you to the datasheet for ab6562, an online copy of the SDS may be found there as well:https://www.abcam.com/protease-inhibitor-cocktail-ab65621.html


I look forward to hearing from you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

This product is provided as a lyophilized powder. You have to reconstitute it in 250 µl of DMSO. This makes it 250 µl of 500X inhibitor cocktail. Which means that for every 499 or 500 µl of the lysate you can add 1 µl of the inhibitor. This means that the total amount of this inhibitor i.e., 250 µl is enough for 500 x 250 µl of the lysate, which is 125 ml of lysate.

Therefore 500 is the dilution factor, I am not sure what the weight means.

Here are some helpful references:


K271 :: Protease Inhibitor Cocktail

- Beaupre, D.M., et al. (2004) Mol. Cancer Ther. 3:179-186.

- Dabkowski, E. et al. Mitochondrial Dysfunction in the Type 2 Diabetic Heart Is Associated with Alterations in Spatially Distinct Mitochondrial Proteosomes.Am. J. Physiol. Heart Circ. Physiol.,2010;299: H529-H540.

- Janckila, A. J., et al. (2005) J. Leukoc. Biol. 77: 209-218.

- Lange S et al (2009) Cardiovasc Res; 81: 159-168.

- Mori Y et al. (2007) J. Virol. 81: 8477-8487.

- Narumi Hashikawa-Hobara et al., Histamine 3 Receptor Activation Reduces the Expression of Neuronal Angiotensin II Type 1 Receptors in the Heart,http://jpet.aspetjournals.org/content/340/1/185.abstract?maxtoshow=&HITS=10&hits=60&RESULTFORMAT=1&andorexacttitle=and&andorexacttitleabs=and&fulltext=BioVision&andorexactfulltext=and&searchid=1&FIRSTINDEX=0&sortspec=match&fdate=1/1/2012&resourcetype=HWCIT

- Ryu M-S et al (2008) J. Nutr.; 138: 2076 - 2083.

- Yun, H. et al. Physical Interaction of Jab1 with Human Serotonin 6 G-protein-coupled Receptor and Their Possible Roles in Cell Survival. J. Biol. Chem. 2010 285: 10016-10029.

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