For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
I have an enquiry regarding a product that we ordered from you, protease cocktail inhibitors, and catalogue number ab65621. It is related to the Method of preparation? As it just said it is enough for 500 tests without mentioning weight and dilution factors!!!!! Please let me know if you have any useful ideas.
Asked on Aug 06 2012
This product is provided as a lyophilized powder. You have to reconstitute it in 250 µl of DMSO. This makes it 250 µl of 500X inhibitor cocktail. Which means that for every 499 or 500 µl of the lysate you can add 1 µl of the inhibitor. This means that the total amount of this inhibitor i.e., 250 µl is enough for 500 x 250 µl of the lysate, which is 125 ml of lysate.
Therefore 500 is the dilution factor, I am not sure what the weight means.
Here are some helpful references:
K271 :: Protease Inhibitor Cocktail
- Beaupre, D.M., et al. (2004) Mol. Cancer Ther. 3:179-186.
- Dabkowski, E. et al. Mitochondrial Dysfunction in the Type 2 Diabetic Heart Is Associated with Alterations in Spatially Distinct Mitochondrial Proteosomes.Am. J. Physiol. Heart Circ. Physiol.,2010;299: H529-H540.
- Janckila, A. J., et al. (2005) J. Leukoc. Biol. 77: 209-218.
- Lange S et al (2009) Cardiovasc Res; 81: 159-168.
- Mori Y et al. (2007) J. Virol. 81: 8477-8487.
- Narumi Hashikawa-Hobara et al., Histamine 3 Receptor Activation Reduces the Expression of Neuronal Angiotensin II Type 1 Receptors in the Heart,http://jpet.aspetjournals.org/content/340/1/185.abstract?maxtoshow=&HITS=10&hits=60&RESULTFORMAT=1&andorexacttitle=and&andorexacttitleabs=and&fulltext=BioVision&andorexactfulltext=and&searchid=1&FIRSTINDEX=0&sortspec=match&fdate=1/1/2012&resourcetype=HWCIT
- Ryu M-S et al (2008) J. Nutr.; 138: 2076 - 2083.
- Yun, H. et al. Physical Interaction of Jab1 with Human Serotonin 6 G-protein-coupled Receptor and Their Possible Roles in Cell Survival. J. Biol. Chem. 2010 285: 10016-10029.
Answered on Aug 06 2012