Product nameProtease Inhibitor Cocktail (EDTA-free)
Abcam’s protease inhibitor cocktails inhibit a variety of endogenous proteases to protect your protein samples from degradation.
Ideal for mammalian cell/tissue lysates and bacterial cell extracts using metal chelation chromatography.
Target proteases - serine proteases, aminopeptidase B, leucine aminopeptidase, cysteine proteases, aspartic proteases
Inhibitors included - AEBSF hydrochloride, aprotinin (bovine lung), bestatin, E-64, leupeptin hemisulfate, pepstatin A
Form: Supplied in DMSO (1 ml)
Preparation: The recommended starting dilution is 1:200
Visit our inhibitor cocktails page for more information on our complete range of convenient and easy to use protease and phosphatase inhibitor cocktails.
Individual components and concentrations:
AEBSF hydrochloride: serine protease inhibitor (100 mM)
Aprotinin (bovine lung): serine protease inhibitor (0.08 mM)
Bestatin: aminopeptidase B and leucine aminopeptidase inhibitor (5 mM)
E-64: cysteine protease inhibitor (1.5 mM)
Leupeptin hemisulfate: serine/cysteine protease inhibitor (2 mM)
Pepstatin A: aspartic protease inhibitor (1 mM)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 ml Protease Inhibitor Cocktail 1 x 1ml
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab201111 has been referenced in 4 publications.
- Xiao G et al. Transferrin 1 Functions in Iron Trafficking and Genetically Interacts with Ferritin in Drosophila melanogaster. Cell Rep 26:748-758.e5 (2019). PubMed: 30650364
- Michalek K & Grabowska M Investigating cellular location of aquaporins in the bovine kidney. A new view on renal physiology in cattle. Res Vet Sci 125:162-169 (2019). PubMed: 31233960
- Song M et al. Trimetazidine restores the positive adaptation to exercise training by mitigating statin-induced skeletal muscle injury. J Cachexia Sarcopenia Muscle 9:106-118 (2018). IF . PubMed: 29152896
- Wu X et al. Baicalin inhibits PDGF-BB-induced hepatic stellate cell proliferation, apoptosis, invasion, migration and activation via the miR-3595/ACSL4 axis. Int J Mol Med 41:1992-2002 (2018). PubMed: 29393361