Product nameProtease Inhibitor Cocktail III
Abcam’s protease inhibitor cocktails inhibit a variety of endogenous proteases to protect your protein samples from degradation.
Ideal for bacterial cell extracts using metal chelation chromatography.
Target proteases - serine proteases, aminopeptidase B, leucine aminopeptidase, cysteine proteases, metalloproteases, aspartic proteases
Inhibitors included - AEBSF hydrochloride, bestatin, E-64, EDTA disodium salt, pepstatin A
Form: Supplied as 1 vial of lyophilised cocktail and 1 vial of DMSO (1 ml)
Preparation: Reconstitute with 1 ml DMSO and 4 ml DI water to obtain a 5 ml solution
Visit our inhibitor cocktails page for more information on our complete range of convenient and easy to use protease and phosphatase inhibitor cocktails.
Individual components and concentrations:
AEBSF hydrochloride: serine protease inhibitor (20 mM)
Bestatin: aminopeptidase B and leucine aminopeptidase inhibitor (1.7 mM)
E-64: cysteine protease inhibitor (0.2 mM)
EDTA disodium salt: metalloprotease inhibitor (85 mM)
Pepstatin A: aspartic protease inhibitor (2 mM)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 kit DMSO 1 x 1ml Protease Inhibitor Cocktail III 1 vial
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab201117 has not yet been referenced specifically in any publications.