Overview

  • Product name
    Anti-Proteasome 19S S5A / ASF antibody
    See all Proteasome 19S S5A / ASF primary antibodies
  • Description
    Rabbit polyclonal to Proteasome 19S S5A / ASF
  • Host species
    Rabbit
  • Specificity
    Detects proteasome 19S subunit S5A.
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Other Immunogen Type corresponding to Human Proteasome 19S S5A/ ASF. Human recombinant proteasome 19S subunit S5A.

  • Positive control
    • HeLa cell lysate
  • General notes

    Previously labelled as Proteasome 19S S5A. 

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 40 kDa).

By Western blot, this antibody detects a 50 kDa protein representing proteasome 19S subunit S5A in HeLa cell lysate.

IP Use at an assay dependent concentration.

Target

  • Function
    Binds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion.
  • Sequence similarities
    Belongs to the proteasome subunit S5A family.
    Contains 2 UIM (ubiquitin-interacting motif) repeats.
    Contains 1 VWFA domain.
  • Domain
    The 2 UIM motifs are involved in the binding to a multi-ubiquitin chain in a cooperative way.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • 26S protease subunit S5a antibody
    • 26S proteasome non ATPase regulatory subunit 4 antibody
    • 26S proteasome non-ATPase regulatory subunit 4 antibody
    • 26S proteasome regulatory subunit rpn10 antibody
    • 26S proteasome regulatory subunit S5A antibody
    • AF 1 antibody
    • AF antibody
    • AF1 antibody
    • Angiocidin antibody
    • Antisecretory factor 1 antibody
    • ASF antibody
    • DS5a antibody
    • MCB 1 antibody
    • MCB1 antibody
    • Multiubiquitin chain binding protein antibody
    • Multiubiquitin chain-binding protein antibody
    • OTTHUMP00000059963 antibody
    • Prosome macropain antibody
    • Proteasome (prosome macropain) 26S subunit non ATPase 4 antibody
    • Proteasome 19S S5A antibody
    • Proteasome 26S non ATPase subunit 4 antibody
    • Proteasome 26S subunit non ATPase 4 antibody
    • PSMD 4 antibody
    • Psmd4 antibody
    • PSMD4_HUMAN antibody
    • pUB R5 antibody
    • pUBR5 antibody
    • Rpn 10 antibody
    • Rpn10 antibody
    • RPN10 homolog antibody
    • S5A antibody
    • S5a/antisecretory factor protein antibody
    see all

Images

  • Western blot of Proteasome 19S S5A / ASF on HeLa cell lysate using ab3318.

References

ab3318 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Regarding your inquiry, this product anti-Proteasome 19S S5A antibody (ab3318) was created against recombinant human proteasome 19S subunit S5A (http://www.expasy.org/uniprot/P55036). The sequence for this protein is: 10 20 30 40 50 60 MVLESTMVCV DNSEYMRNGD FLPTRLQAQQ DAVNIVCHSK TRSNPENNVG LITLANDCEV 70 80 90 100 110 120 LTTLTPDTGR ILSKLHTVQP KGKITFCTGI RVAHLALKHR QGKNHKMRII AFVGSPVEDN 130 140 150 160 170 180 EKDLVKLAKR LKKEKVNVDI INFGEEEVNT EKLTAFVNTL NGKDGTGSHL VTVPPGPSLA 190 200 210 220 230 240 DALISSPILA GEGGAMLGLG ASDFEFGVDP SADPELALAL RVSMEEQRQR QEEEARRAAA 250 260 270 280 290 300 ASAAEAGIAT TGTEDSDDAL LKMTISQQEF GRTGLPDLSS MTEEEQIAYA MQMSLQGAEF 310 320 330 340 350 360 GQAESADIDA SSAMDTSEPA KEEDDYDVMQ DPEFLQSVLE NLPGVDPNNE AIRNAMGSLA 370 SQATKDGKKD KKEEDKK I hope that this information is helpful, and please let me know if you have any additional questions.

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Answer

Thank you for your enquiry regarding ab3318. This antibody is a rabbit polyclonal antibody raised against the human recombinant full length proteasome 19S S5A protein. It will therefore recognise multiple epitopes on the protein. The antibody is therefore likely to contain IgGs which recognise a portion only of the protein and therefore I would expect it to recognise your truncated protein. However as this has not been tested I cannot guarantee it. I hope this information will help you in your choice of antibody,

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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